tudy of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) comple

Autor(es):Mostrar menos -

Aleixo, Nadia Andrade ^[1] ; Gomes, Pietra Stefany da Silva ^[1] ; da Silva, Patricia Bento ^{[2, 3]} ; Sato, Mariana Rillo ^[2] ; Campos, Debora Leite ^[4] ; Barud, Hernane da Silva ^[1] ; Castro, Guillermo Raul ^{[5, 6]} ; Islan, German Abel ^[5] ; Toledo, Constanza ^[5] ; Karp, Federico ^[7] ; Chorilli, Marlus ^[2] ; Pavan, Fernando Rogerio ^[4] ; Resende, Flavia Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Araraquara UNIARA, Dept Biol Sci & Hlth, Araraquara - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Sao Paulo - Brazil

^[3] Univ Brasilia, Nanobiotechnol Lab, Inst Biol Sci, Dept Genet & Morphol, Brasilia, DF - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo - Brazil

^[5] Univ Nacl Plata CONICET CCT Plata, Dept Quim, Lab Nanobiomat, Fac Ciencias Exactas, La Plata, Buenos Aires - Argentina

^[6] Univers Nacl Rosario, Partner Lab Max Planck Inst Biophys Chem MPIbpC, Max Planck Lab Struct Biol Chem & Mol Biophys Ro, Ctr Estudios Interdisciplinarios CEI, Rosario - Argentina

^[7] Univ Nacl Litoral UNL, Lab Quim Fina UNL CONICET, INTEC, Santa Fe - Argentina

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microencapsulation; v. 39, n. 1, p. 61-71, JAN 2 2022.
• Citações Web of Science: 0

Resumo

This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)(2)center dot H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit (R), Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and -24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and -6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity. (AU)

• Processo FAPESP: 13/09265-7 - Avaliação do potencial de sistemas lipídicos nanoestruturados para administração de compostos de Cu(II) aplicáveis na otimização da terapia da tuberculose
• Beneficiário: Patrícia Bento da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/16278-9 - Estudo do potencial genotoxicológico e da permeabilidade em células Caco-2 de complexos metálicos com promissoras atividades biológicas
• Beneficiário: Flávia Aparecida Resende Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular