tudy of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) comple

Author(s):Show less -

Aleixo, Nadia Andrade ^[1] ; Gomes, Pietra Stefany da Silva ^[1] ; da Silva, Patricia Bento ^{[2, 3]} ; Sato, Mariana Rillo ^[2] ; Campos, Debora Leite ^[4] ; Barud, Hernane da Silva ^[1] ; Castro, Guillermo Raul ^{[5, 6]} ; Islan, German Abel ^[5] ; Toledo, Constanza ^[5] ; Karp, Federico ^[7] ; Chorilli, Marlus ^[2] ; Pavan, Fernando Rogerio ^[4] ; Resende, Flavia Aparecida ^[1]

Affiliation

^[1] Univ Araraquara UNIARA, Dept Biol Sci & Hlth, Araraquara - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Sao Paulo - Brazil

^[3] Univ Brasilia, Nanobiotechnol Lab, Inst Biol Sci, Dept Genet & Morphol, Brasilia, DF - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo - Brazil

^[5] Univ Nacl Plata CONICET CCT Plata, Dept Quim, Lab Nanobiomat, Fac Ciencias Exactas, La Plata, Buenos Aires - Argentina

^[6] Univers Nacl Rosario, Partner Lab Max Planck Inst Biophys Chem MPIbpC, Max Planck Lab Struct Biol Chem & Mol Biophys Ro, Ctr Estudios Interdisciplinarios CEI, Rosario - Argentina

^[7] Univ Nacl Litoral UNL, Lab Quim Fina UNL CONICET, INTEC, Santa Fe - Argentina

• Document type: Journal article
• Source: Journal of Microencapsulation; v. 39, n. 1, p. 61-71, JAN 2 2022.
• Web of Science Citations: 0

Abstract

This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)(2)center dot H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit (R), Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and -24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and -6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity. (AU)

• FAPESP's process: 13/09265-7 - Evaluation of the potential nanostructured lipid systems for administration of Cu(II) compounds applicable in optimizing the treatment of tuberculosis
• Grantee: Patrícia Bento da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/16278-9 - Study of the genotoxicological potential and permeation assay with Caco-2 cells of metallic complexes with promising biological activities
• Grantee: Flávia Aparecida Resende Nogueira
• Support Opportunities: Regular Research Grants