Development and evaluation of pre-clinical safety of polymeric nanostructures for encapsulation of photolyase

• Grant number: 16/03887-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): September 01, 2016
• Effective date (End): May 14, 2019
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Carlota de Oliveira Rangel Yagui
• Grantee: Camila Areias de Oliveira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In 2015, non-melanoma skin cancer (basal cell carcinoma and squamous cell carcinoma) was the highest incidence in the Brazilian population. Ultraviolet (UV) radiation is the main cause of this cancer and estimates point that 182,000 people in Brazil received this diagnosis in 2015, reinforcing the problem. Topical application of DNA repair enzymes, such as photolyase, is a convenient strategy against damage induced by UV radiation and may reduce photocarcinogenesis. However, for the enzyme to exert biological activity, it must reach the site of action. Thus, the development of polymeric nanostructures for encapsulation of photolyase is adequate and an effective approach for use in topical formulations. Therefore, the aim of this work is to develop and evaluate the pre-clinical safety of polymeric nanostructures for encapsulation of photolyase. The specific goals will be: (i) to develop polymeric nanostructures for encapsulation of photolyase, (ii) to perform the physical, physico-chemical and morphological characterization of the nanostructures, (III) to determine the enzymatic activity of photolyase, (IV) to assess the release profile of the photolyase from the nanostructures, (V) to assess in vitro the cytotoxicity profile of the nanoparticles, (VI) to analyze the in vitro penetration / permeation of the nanostructures, (VII) to analyze in vitro the irritation potential with the HET-CAM method. We hope that the results present possibilities for practical application in strategic areas of photoprotective cosmetics development associated with chemopreventive enzymes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

APOLINARIO, ALEXSANDRA CONCEICAO; FERRARO, RAFAEL BERTELLI; DE OLIVEIRA, CAMILA AREIAS; PESSOA, JR., ADALBERTO; RANGEL-YAGUI, CARLOTA DE OLIVEIRA. Quality-by-Design Approach for Biological API Encapsulation into Polymersomes Using ``Off-the-Shelf{''} Materials: a Study on L-Asparaginase. AAPS PHARMSCITECH, v. 20, n. 6, AUG 2019. (17/03811-0, 13/08617-7, 16/03887-4, 14/10456-4)

OLIVEIRA, CAMILA AREIAS; FORSTER, CAMILA; LEO, PATRICIA; RANGEL-YAGUI, CARLOTA. Development of triblock polymersomes for catalase delivery based onquality by designenvironment. JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, v. 43, n. 1, p. 126-135, DEC 22 2021. (16/03887-4, 16/22065-5)