Toxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load levels

Autor(es)

Gabriela Rodrigues e Fonseca ^[1] ; Marcelo Andreetta Corral ^[2] ; Fabiana Martins de Paula ; Dirce Mary Correia Lima Meisel ^[4] ; Ronaldo Cesar Borges Gryschek ; Susana Angélica Zevallos Lescano

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 64, 2022-02-02.

Resumo

ABSTRACT The Western-blotting technique was applied to identify antigenic fractions of excretory-secretory Toxocara canis antigen recognized by IgG antibodies throughout an experimental infection in mice challenged by different inocula. Mice were inoculated with 5, 50 and 500 embryonated eggs and serum samples were collected 15, 30, 60, 90 and 120 days post-infection. Serum samples were analyzed using an excretory-secretory Toxocara antigen. Antibodies recognized antigenic fractions from 30 to 90 kDa. The protein fraction of 30-35 kDa was the most frequently recognized regardless of the size of inoculum and the stage of infection represented by the different collection times, but the antigenic recognition was more evident in groups infected with 50 and 500 eggs. This study presents an antigenic panel of the excretory-secretory antigen of T. canis and suggests that the 30-35 kDa antigenic fraction is a promising marker of the infection and should be further explored in future studies on experimental toxocariasis. (AU)

• Processo FAPESP: 14/07345-6 - PCR para detecção de DNA de Toxocara canis em amostras de soro de camundongos experimentalmente infectados, comparação com técnicas imunológicas
• Beneficiário: Susana Angélica Zevallos Lescano
• Modalidade de apoio: Auxílio à Pesquisa - Regular