Autor(es):Mostrar menos -

De Rossi, Hugo ; Bortoliero Costa, Camila ; Rodrigues-Rossi, Luana Teixeira ; Barros Nunes, Giovana ; Spinosa Cheles, Doris ; Maran Pereira, Isabella ; Rocha, Daniele F. O. ; Feitosa, Eloi ; Colnaghi Simionato, Ana Valeria ; Zoccal Mingoti, Gisele ; Benites Aoki, Pedro Henrique ; Gouveia Nogueira, Marcelo Fabio

• Tipo de documento: Artigo Científico
• Fonte: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY; v. 50, n. 1, p. 10-pg., 2022-12-31.

Resumo

The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48 h of incubation at 38.5 degrees C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48 h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0 mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner. (AU)

• Processo FAPESP: 20/07634-9 - Predição de batimento cardíaco fetal por meio de inteligência artificial e de variáveis morfológicas, morfocinéticas e relacionadas com a paciente
• Beneficiário: Dóris Spinosa Chéles
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 20/11596-5 - O impacto da suplementação de bFGF, no cultivo in vitro de embriões bovinos, sobre a diferenciação da massa celular interna
• Beneficiário: Isabella Maran Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/50533-2 - GIFT: melhoramento genômico de características relacionadas com a fertilização em gado bovino dinamarquês e brasileiro
• Beneficiário: Marcelo Fábio Gouveia Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/10732-5 - O uso do peptídeo natriurético tipo-C no cultivo in vitro de embriões bovinos altera as características in vitro e in vivo de sua criotolerância?
• Beneficiário: Camila Bortoliero Costa Giovannetti
• Modalidade de apoio: Bolsas no Brasil - Doutorado