| Full text | |
| Author(s): Show less - |
De Rossi, Hugo
;
Bortoliero Costa, Camila
;
Rodrigues-Rossi, Luana Teixeira
;
Barros Nunes, Giovana
;
Spinosa Cheles, Doris
;
Maran Pereira, Isabella
;
Rocha, Daniele F. O.
;
Feitosa, Eloi
;
Colnaghi Simionato, Ana Valeria
;
Zoccal Mingoti, Gisele
;
Benites Aoki, Pedro Henrique
;
Gouveia Nogueira, Marcelo Fabio
Total Authors: 12
|
| Document type: | Journal article |
| Source: | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY; v. 50, n. 1, p. 10-pg., 2022-12-31. |
| Abstract | |
The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48 h of incubation at 38.5 degrees C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48 h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0 mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner. (AU) | |
| FAPESP's process: | 20/07634-9 - Prediction of fetal heartbeat through artificial intelligence and morphological, morphokinetic and patient-related variables |
| Grantee: | Dóris Spinosa Chéles |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 20/11596-5 - The impact of bFGF supplementation in in vitro culture of bovine embryos at inner cell mass differentiation |
| Grantee: | Isabella Maran Pereira |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle |
| Grantee: | Marcelo Fábio Gouveia Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/10732-5 - Does the use of natriuretic peptide type-C in in vitro culture of bovine embryos alter the in vitro and in vivo characteristics of its criotolerance? |
| Grantee: | Camila Bortoliero Costa Giovannetti |
| Support Opportunities: | Scholarships in Brazil - Doctorate |