Antimicrobial Resistance and Molecular Characterization of Staphylococcus aureus Recovered from Cows with Clinical Mastitis in Dairy Herds from Southeastern Brazil

Autor(es)

Freu, Gustavo ; Tomazi, Tiago ; Filho, Antonio F. S. ; Heinemann, Marcos B. ; dos Santos, Marcos, V

• Tipo de documento: Artigo Científico
• Fonte: ANTIBIOTICS-BASEL; v. 11, n. 4, p. 11-pg., 2022-04-01.

Resumo

Staphylococcus aureus is a contagious pathogen frequently associated with bovine mastitis in Brazil. Molecular characterization of Staph. aureus isolated from affected mammary quarters of cows with clinical mastitis (CM) can provide data on epidemiological behavior of this pathogen and antimicrobial susceptibility (AMS) assessment at the genotypic level. This study genotypically characterized Staph. aureus isolates recovered from cows with CM and determined the association of genotypes and AMS. A total of 84 Staph. aureus strains identified from affected mammary quarters of cows with CM in 13 dairy herds from Southeastern Brazil were submitted for susceptibility testing to 10 antimicrobials using the technique of minimal inhibitory concentration. The same isolates were also genotyped using the spa-typing methodology. Results showed a high genotypic similarity between the Staph. aureus isolates within and between herds, which were categorized as resistant to most antimicrobials, especially to beta-lactam antibiotics. In addition, differences in AMS were observed among genotypic clusters, which may affect the efficacy of antimicrobials used to treat CM in different dairy herds. (AU)

• Processo FAPESP: 13/22286-3 - Perfil de agentes etiológicos causadores de mastite clínica e uso de antimicrobianos em rebanhos leiteiros
• Beneficiário: Tiago Tomazi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/17411-6 - Impacto econômico e perfil de agentes etiológicos da mastite bovina
• Beneficiário: Marcos Veiga dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/10332-6 - Estudo da diversidade molecular de linhagens de Staphylococcus aureus isolados de mastite bovina
• Beneficiário: Marcos Bryan Heinemann
• Modalidade de apoio: Auxílio à Pesquisa - Regular