Glutamine supplementation improves contractile function of regenerating soleus muscles from rats

Autor(es)

Koike, Tatiana E. ; Dell Aquila, Rodrigo A. ; Silva, Kellana S. ; Aoki, Marcelo S. ; Miyabara, Elen H.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY; v. 43, n. 2, p. 11-pg., 2022-02-24.

Resumo

This study evaluated the effects of glutamine supplementation immediately after freezing injury on morphological and contractile function of regenerating soleus muscles from rats. Young male Wistar rats were subjected to cryolesion of soleus muscles, and immediately after received a daily supplementation of glutamine (1 g/kg/day). The muscles were evaluated on post-injury days 3 and 10. Glutamine-supplemented injured muscles had a lower number of CD11b positive immune cells and higher mRNA levels of IL-4 compared to those from the cryolesioned muscles analyzed on post-injury day 3. The mRNA and protein expression levels of the myogenic transcription factor MyoD were also higher in glutamine-supplemented injured muscles than in injured muscles examined on post-cryolesion day 3. In addition, glutamine-supplemented injured muscles had a higher size of their regenerating myofibers, attenuated decline in maximum tetanic strength and improved fatigue resistance compared to those from injured muscles evaluated on post-cryolesion day 10. No effect was observed in uninjured muscles supplemented with glutamine. Our results suggest that glutamine supplementation improves the resolution of inflammation, as well as the size and functional recovery of regenerating myofibers from soleus muscles by accelerating the up-regulation of IL-4 and MyoD expression. Future non-pharmacological rehabilitation studies are warranted to investigate the effect of glutamine supplementation on the outcome of injured skeletal muscles. (AU)

• Processo FAPESP: 17/09069-4 - Influência do adrenoceptor beta 2 na função das células satélite
• Beneficiário: Tatiana Emy Koike
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/23391-8 - Resposta regenerativa da musculatura esquelética de camundongos com síndrome de Marfan
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/24946-4 - Reabilitação do músculo esquelético de camundongos com síndrome de Marfan: efeito do exercício excêntrico
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular