Salmonella Typhimurium O-antigen and VisP play an important role in swarming and osmotic stress response during intracellular conditions

Autor(es)

Manieri, Fernanda Z. ; Moreira, Cristiano G.

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 53, n. 2, p. 8-pg., 2022-03-18.

Resumo

Salmonella Typhimurium is a pathogen of clinical relevance and a model of study in host-pathogen interactions. The virulence and stress-related periplasmic protein VisP is important during S. Typhimurium pathogenesis. It supports bacteria invading host cells, surviving inside macrophages, swimming, and succeeding in murine colitis model, O-antigen assembly, and responding to cationic antimicrobial peptides. This study aimed to investigate the role of the O-antigen molecular ruler WzzST and the periplasmic protein VisP in swarming motility and osmotic stress response. Lambda red mutagenesis was performed to generate single and double mutants, followed by swarming motility, qRT-PCR, Western blot, and growth curves. Here we demonstrate that the deletion of visP affects swarming under osmotic stress and changes the expression levels of genes responsible for chemotaxis, flagella assembly, and general stress response. The deletion of the gene encoding for the O-antigen co-polymerase wzzST increases swarming motility but not under osmotic stress. A second mutation in O-antigen co-polymerase wzzST in a Delta visP background affected gene expression levels. The Delta visP growth was affected by sodium and magnesium levels on N-minimum media. These data indicate that WzzST has a role in swarming the motility of S. Typhimurium, as the VisP is involved in chemotaxis and osmotic stress, specifically in response to MgCl2 and NaCl. (AU)

• Processo FAPESP: 19/03049-7 - Transcriptoma da sinalização química bacteriana em Salmonella Typhimurium, investigação de enteropatógenos bacterianos globais emergentes em São Paulo e emprego de modelo alternativo de infecção no estudo da patogênese.
• Beneficiário: Cristiano Gallina Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular