The dimerization of methyl vanillate improves its effect against breast cancer cells via pro-oxidant effect

Autor(es)

Pessoa, Adriano de Souza ; Tokuhara, Cintia Kazuko ; Fakhoury, Vanessa Svizzero ; Pagnan, Ana Ligia ; de Oliveira, Gabriela Silva Neubern ; Sanches, Mariana Liessa Rovis ; Inacio, Kelly Karina ; Costa, Bruna Carolina ; Ximenes, Valdecir Farias ; de Oliveira, Rodrigo Cardoso

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 361, p. 9-pg., 2022-07-01.

Resumo

Phenolic phytochemicals are a group of organic compounds with potent antioxidant features but can also act as powerful pro-oxidants. These characteristics are effective in reducing metastatic potential in cancer cells, and this effect has been associated with reactive oxygen species (ROS). Methyl vanillate (MV) and its dimer, methyl divanillate (DMV), are potent antioxidants. In the present study, we investigated the effects of MV and DMV on breast cancer cell lines MCF-7 and MDA-MB-231 and compared the results using the non-tumor cell line HB4a. Our results indicated that the compounds performed a pro-oxidant action, increasing the generation of ROS. DMV decreased the viability cell, showing a higher apoptotic effect and inhibition of proliferation than MV on both cell lines, with significant differences between groups (p < 0.05). Some modulation of NOX4, NOX5, and DUOX were observed, but the results did not correlate with the intracellular production of ROS. The dimer showed more effectivity and pro-oxidant effect than MV, impacting cell line MCF-7 in higher extension than MDA-MB-231. In conclusion, and corroborating with reported works, the dimerization of natural phenolic compounds was associated with improved beneficial biological effects as a potential cytotoxic agent to tumor cells. (AU)

• Processo FAPESP: 18/12607-0 - Confecção e avaliação, in vivo e in vitro, de um construto composto por células-tronco mesenquimais derivadas do tecido adiposo, biomateriais substitutos ósseos e plasma rico em fibrinas.
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/08890-3 - Estudo do envolvimento do leucotrieno B4 no controle do processo de angiogênese acoplado à osteogênese na condição de artrite.
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/26309-9 - Efeito do vanilato de metila e divanilato de metila em células de câncer de mama: regulação do complexo NADPH oxidase
• Beneficiário: Adriano de Souza Pessoa
• Modalidade de apoio: Bolsas no Brasil - Mestrado