Use of 2D and co-culture cell models to assess the toxicity of zein nanoparticles loading insect repellents icaridin and geraniol

Autor(es)

Germano-Costa, T. ; Bilesky-Jose, N. ; Guilger-Casagrande, M. ; Pasquoto-Stigliani, T. ; Rogerio, CB. ; Abrantes, DC. ; Maruyama, CR. ; Oliveira, JL. ; Fraceto, LF. ; Lima, R.

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 216, p. 12-pg., 2022-08-01.

Resumo

After the latest dengue and Zika outbreaks, the fight against mosquito vectors has become an emerging area of research. One tool for this combat is repellents; however, these products are composed of different toxic agents. Botanical compounds with repellent potential are an alternative; however these compounds are highly volatile. Thus, the present study aimed to synthesize zein-based polymeric nanoparticles as an efficient carrier system for the sustained release of the repellents icaridin and geraniol and evaluate the toxicity of these nanorepellents comparing two different cell models. In vitro tests were carried out due to current Brazilian legislation pro-hibiting animal testing for cosmetics (current classification of repellents). The cytotoxicity and genotoxicity of the nanoparticles were evaluated in 2D and co-culture cell models (A549/lung epithelium, HaCaT/keratinocytes, HT-29/intestinal epithelium, and THP-1/peripheral blood monocytes). Cell viability by mitochondrial activity, cell membrane integrity, damage to genetic material, and expression of genes involved in the allergic/inflam-matory system were evaluated. The results of cytotoxicity evaluation showed cell viability above 70% in both cell models. No differences were observed in genotoxicity assessment between cells exposed to nanorepellents and controls. In contrast, gene expression analysis showed increased cytokine expression for the emulsion compounds in 2D cell cultures compared to co-cultures. These findings open perspectives that zein-based nanorepellents have potential applications due to the reduced toxicity observed when the compounds are encapsulated and emerge as an alternative for arbovirus control. In addition, the study demonstrated that depending on the analysis, different results might be observed when comparing 2D and co-culture cell models to evaluate the toxicity of new nanosystems. (AU)

• Processo FAPESP: 20/05816-2 - Estudo do recobrimento (capping) de nanopartículas biogênicas e sua correlação com toxicidade e atividade no controle de fitopatógenos
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/20932-6 - Síntese verde e avaliação de nanopartículas de ferro baseadas em Trichoderma harzianum visando aplicação na agricultura
• Beneficiário: Natalia Bilesky José
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/14734-0 - Repelentes de longa duração contendo misturas de ativos em associação com carreadores lipídicos nanoestruturados para combate à propagação de arboviroses
• Beneficiário: Daniele Carvalho Abrantes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/18231-4 - Micropartículas poliméricas como sistema carreador do fungo Trichoderma harzianum visando aplicações em agricultura
• Beneficiário: Cintia Rodrigues Maruyama
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/25702-9 - Avaliação in vitro da toxicidade de sistemas carreadores para o desenvolvimento de repelentes de longa duração em modelos 2D, co-cultura e 3D
• Beneficiário: Tais Germano da Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/02404-5 - Hidrogéis contendo os repelentes geraniol e icaridina encapsulados em nanopartículas de zeína como alternativa para combate a arboviroses
• Beneficiário: Carolina Barbara Rogerio
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica