Photobiomodulation treatments drive osteogenic versus adipocytic fate of bone marrow mesenchymal stem cells reversing the effects of hyperglycemia in diabetes

Autor(es)

Bueno, Natalia Pieretti ; Kfouri, Cecilia Cardoso ; Copete, Isabella Nunes ; de Oliveira, Fabiola Singaretti ; Arany, Praveen ; Marques, Marcia Martins ; Ferraz, Emanuela Prado

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. N/A, p. 10-pg., 2022-04-02.

Resumo

Diabetes mellitus (DM) is a chronic metabolic disease that affects bone metabolism, which can be related to a reduced osteogenic potential of bone marrow mesenchymal stem cells (BM-MSCs). MSCs from diabetic rats (dBM-MSC) have shown a tendency to differentiate towards adipocytes (AD) instead of osteoblasts (OB). Since photobiomodulation (PBM) therapy is a non-invasive treatment capable of recovering the osteogenic potential of dBM-MSCs, we aimed to evaluate whether PBM can modulate MSC's differentiation under hyperglycemic conditions. BM-MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB) and adipocytes (AD and dAD). dOB and dAD were treated with PBM every 3 days (660 nm; 5 J/cm(2); 0.14 J; 20 mW; 0.714 W/cm(2)) for 17 days. Cell morphology and viability were evaluated, and cell differentiation was confirmed by gene expression (RT-PCR) of bone (Runx2, Alp, and Opn) and adipocyte markers (Ppar gamma, C/Ebp alpha, and C/Ebp beta), production of extracellular mineralized matrix (Alizarin Red), and lipid accumulation (Oil Red). Despite no differences on cell morphology, the effect of DM on cells was confirmed by a decreased gene expression of bone markers and matrix production of dOB, and an increased expression of adipocyte and lipid accumulation of dAD, compared to heatlhy cells. On the other hand, PBM reversed the effects of dOB and dAD. The negative effect of DM on cells was confirmed, and PBM improved OB differentiation while decreasing AD differentiation, driving the fate of dBM-MSCs. These results may contribute to optimizing bone regeneration in diabetic patients. (AU)

• Processo FAPESP: 18/20302-5 - Efeito da terapia de fotobiomodulação na diferenciação osteoblástica de células-tronco mesenquimais derivadas da medula óssea de ratos diabéticos
• Beneficiário: Isabella Nunes Copete
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 21/04874-1 - Efeito priming da terapia de fotobiomodulação no potencial osteogênico de células-tronco mesenquimais derivadas do tecido adiposo
• Beneficiário: Emanuela Prado Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/23350-3 - Papel da terapia de fotobiomodulação no destino das células-tronco mesenquimais derivadas da medula óssea de ratos diabéticos
• Beneficiário: Cecília Cardoso Kfouri
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica