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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Leukotriene B4 Loaded in Microspheres Inhibits Osteoclast Differentiation and Activation

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Autor(es):
Francine Lorencetti-Silva ; Maya Fernanda Manfrin Arnez [2] ; João Pedro de Queiroz Thomé ; Marcio Santos de Carvalho [4] ; Fabrício Kitazono de Carvalho [5] ; Alexandra Mussolino de Queiroz [6] ; Lúcia Helena Faccioli [7] ; Francisco Wanderley Garcia Paula-Silva
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Brazilian Dental Journal; v. 33, n. 5, p. 35-45, 2022-10-21.
Resumo

Abstract To investigate osteoclast formation in vivo and if leukotriene B4 (LTB4) loaded in microspheres (MS) could be used as a therapeutical strategy to promote a sustained delivery of the mediator and prevent osteoclast differentiation. Methods: In vivo, apical periodontitis was induced in mice to investigate osteoclast differentiation and signaling in absence of 5-lipoxygenase (5-LO). In vitro, LTB4-MS were prepared using an oil-in-water emulsion solvent extraction-evaporation process. Characterization and efficiency of LTB4 encapsulation were investigated. J774A.1 macrophages were cultured in the presence of monocyte colony-stimulating factor (M-CSF) and ligand for receptor activator of nuclear factor kappa B (RANKL) and then stimulated with LTB4-MS. Cytotoxicity, in vitro MS-LTB4 uptake, osteoclast formation and gene expression were measured. Results: We found that 5-LO negatively regulates osteoclastic formation in vivo during apical periodontitis development. In vitro, LTB4-MS were up-taken by macrophages and were not cytotoxic to the cells. LTB4-MS inhibited osteoclast formation and the synthesis of osteoclastogenic genes Acp5, Mmp9, Calcr and Ctsk. LTB4-MS inhibited differentiation of macrophages into an osteoclastic phenotype and cell activation under M-CSF and RANKL stimulus. (AU)

Processo FAPESP: 14/14015-2 - Estudo da diferenciação de células da linhagem hematopoiética via 5-lipoxigenase
Beneficiário:Joao Pedro de Queiroz Thome
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 14/07125-6 - Novos aspectos funcionais dos eicosanóides
Beneficiário:Lúcia Helena Faccioli
Modalidade de apoio: Auxílio à Pesquisa - Temático