| Full text | |
| Author(s): |
Francine Lorencetti-Silva
;
Maya Fernanda Manfrin Arnez
[2]
;
João Pedro de Queiroz Thomé
;
Marcio Santos de Carvalho
[4]
;
Fabrício Kitazono de Carvalho
[5]
;
Alexandra Mussolino de Queiroz
[6]
;
Lúcia Helena Faccioli
[7]
;
Francisco Wanderley Garcia Paula-Silva
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Brazilian Dental Journal; v. 33, n. 5, p. 35-45, 2022-10-21. |
| Abstract | |
Abstract To investigate osteoclast formation in vivo and if leukotriene B4 (LTB4) loaded in microspheres (MS) could be used as a therapeutical strategy to promote a sustained delivery of the mediator and prevent osteoclast differentiation. Methods: In vivo, apical periodontitis was induced in mice to investigate osteoclast differentiation and signaling in absence of 5-lipoxygenase (5-LO). In vitro, LTB4-MS were prepared using an oil-in-water emulsion solvent extraction-evaporation process. Characterization and efficiency of LTB4 encapsulation were investigated. J774A.1 macrophages were cultured in the presence of monocyte colony-stimulating factor (M-CSF) and ligand for receptor activator of nuclear factor kappa B (RANKL) and then stimulated with LTB4-MS. Cytotoxicity, in vitro MS-LTB4 uptake, osteoclast formation and gene expression were measured. Results: We found that 5-LO negatively regulates osteoclastic formation in vivo during apical periodontitis development. In vitro, LTB4-MS were up-taken by macrophages and were not cytotoxic to the cells. LTB4-MS inhibited osteoclast formation and the synthesis of osteoclastogenic genes Acp5, Mmp9, Calcr and Ctsk. LTB4-MS inhibited differentiation of macrophages into an osteoclastic phenotype and cell activation under M-CSF and RANKL stimulus. (AU) | |
| FAPESP's process: | 14/14015-2 - Study of cell differentiation of the hematopoietic lineage via 5-lipoxygenase |
| Grantee: | Joao Pedro de Queiroz Thome |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/07125-6 - New functional aspects of eicosanoids |
| Grantee: | Lúcia Helena Faccioli |
| Support Opportunities: | Research Projects - Thematic Grants |