Hydroxymethylnitrofurazone (NFOH) decreases parasitaemia, parasitism and tissue lesion caused by infection with the Bolivia Trypanosoma cruzi type I strain in Swiss and C57BL/6 mice

Autor(es)

Cauê Benito Scarim ^[1] ; Cleverton Roberto de Andrade ^[2] ; Rossana Falcone ^[3] ; Letícia Moreno Ambrozini ^[4] ; Vitor Izidoro Senhorelli ^[5] ; João Aristeu da Rosa ^[6] ; Chung Man Chin ^[7]

Afiliação do(s) autor(es)

^[1] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of Pharmaceuticals and Medicines - Brasil

^[2] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Dentistry. Department of Physiology and Pathology - Brasil

^[3] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of biosciences and biotechnology - Brasil

^[4] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of biosciences and biotechnology - Brasil

^[5] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of Pharmaceuticals and Medicines - Brasil

^[6] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of biosciences and biotechnology - Brasil

^[7] Sao Paulo State University “Júlio de Mesquita Filho”, UNESP. Faculty of Pharmaceutical Sciences. Department of Pharmaceuticals and Medicines - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Pharmaceutical Sciences; v. 58, 2023-01-09.

Resumo

Abstract The chemical hydroxymethylation of the antimicrobial nitrofurazone leads to the prodrug NFOH, also increases the anti-T. cruzi activities (in vitro and in vivo), as well as showed non-genotoxic (Ames and micronucleus assays). In the present study, we assessed the anti-T. cruzi effect of the NFOH In vivo - in acute Swiss and C57Bl/6 experimental Chagas models. The treatment started at 5 days post-infection during 20 consecutive days (orally, once day, 150mg/kg), and the parasitaemia as well as histopathology analysis were performed. In both experimental murine models, NFOH was able to reduce parasitemia blood avoiding parasitic reactivation, during immunosuppression period (dexamethasone 5mg/kg, 14 days), in 100% of the mice, and decrease tissue parasite nests, demonstrating absence of amastigote forms in all organs (100%) analyzed, data similar to benznidazole (BZN). Therefore, the results shown here pointing to the NFOH as promising compound for further preclinical studies, being a high potential drug to effective and safe chemotherapy to Chagas disease. (AU)

• Processo FAPESP: 16/10847-9 - Hidroximetilnitrofural (NFOH): estudo da atividade em fase crônica da Doença de Chagas em modelo animal e síntese de derivados solúveis
• Beneficiário: Cauê Benito Scarim
• Modalidade de apoio: Bolsas no Brasil - Doutorado