| Texto completo | |
| Autor(es): |
Graminha, Angelica E.
;
Popolin, Cecilia
;
de Araujo-Neto, Joao Honorato
;
Correa, Rodrigo S.
;
de Oliveira, Katia M.
;
Godoy, Luani R.
;
Vegas, Legna Colina
;
Ellena, Javier
;
Batista, Alzir A.
;
Cominetti, Marcia R.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY; v. 243, p. 14-pg., 2022-12-05. |
| Resumo | |
In this work we present the synthesis and characterization of six new ruthenium compounds with general formulae [Ru(L)(dppb)(bipy)]PF6 and [Ru(L)(dppe)2]PF6 where L = salicylic acid (Sal), 4-aminosalicylic acid (AmSal) or 2,4-dihydroxybenzoic acid (DiSal), dppb = 1,4-bis(diphenylphosphino)butane, dppe = 1,2-bis (diphenylphosphino)ethane and bipy = 2,2 '-bipyridine. The complexes were characterized by elemental analysis, molar conductivity, cyclic voltammetry, NMR, UV-vis and IR spectroscopies, and two by X-ray crystallography. The 31P{1H} NMR spectra of the complexes with the general formula [Ru(L)(dppe)2]PF6 showed that the phosphorus signals are solvent-dependent. Aprotic solvents, which form strong hydrogen bonds with the com-plexes, inhibit the free rotation of the salicylic acid-based, modifying the diphosphine cone angles, leading to distortion of the phosphorus signals in the NMR spectra. The cytotoxicity of the complexes was evaluated in MCF-7, MDA-MB-231, SKBR3 human breast tumor cells, and MCF-10 non-tumor cell lines. The complexes with the structural formula [Ru(L)(dppe)2]PF6 were the most cytotoxic, and the complex [Ru(AmSal)(dppe)2]PF6 with L = 4-aminosalicylic acid ligand was the most selective for the MDA-MB-231 cell line. This complex interacts with the transferrin and induces apoptosis through the intrinsic pathway, as demonstrated by increased levels of proteins involved in apoptotic cell death. (AU) | |
| Processo FAPESP: | 17/15850-0 - Difração de raios X como ferramenta no desenvolvimento de potenciais fármacos |
| Beneficiário: | Eduardo Ernesto Castellano |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 14/19632-0 - Novos complexos de rutênio com ligantes bioativos: investigação do mecanismo de ação |
| Beneficiário: | Angelica Ellen Graminha |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/04876-4 - Estudos sobre estrutura & atividade de complexos de RuII/areno/mercaptoligantes frente ao câncer |
| Beneficiário: | João Honorato de Araujo Neto |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 20/14561-8 - Estudo in vitro e in vivo de complexos fosfínicos de Ru(II) com atividades anticancerígenas |
| Beneficiário: | Alzir Azevedo Batista |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |