ArtinM Cytotoxicity in B Cells Derived from Non-Hodgkin's Lymphoma Depends on Syk and Src Family Kinases

Autor(es)

Barboza, Bruno Rafael ; Thomaz, Sandra Maria de Oliveira ; de Carvalho, Airton ; Espreafico, Enilza Maria ; Miyamoto, Jackson Gabriel ; Tashima, Alexandre Keiji ; Camacho, Mauricio Frota ; Zelanis, Andre ; Roque-Barreira, Maria Cristina ; da Silva, Thiago Aparecido

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 2, p. 24-pg., 2023-01-01.

Resumo

Receptors on the immune cell surface have a variety of glycans that may account for the immunomodulation induced by lectins, which have a carbohydrate recognition domain (CRD) that binds to monosaccharides or oligosaccharides in a specific manner. ArtinM, a D-mannose-binding lectin obtained from Artocarpus heterophyllus, has affinity for the N-glycans core. Immunomodulation by ArtinM toward the Th1 phenotype occurs via its interaction with TLR2/CD14 N-glycans on antigen-presenting cells, as well as recognition of CD3 gamma N-glycans on murine CD4+ and CD8+ T cells. ArtinM exerts a cytotoxic effect on Jurkat human leukemic T-cell line and human myeloid leukemia cell line (NB4). The current study evaluated the effects of ArtinM on murine and human B cells derived from non-Hodgkin's lymphoma. We found that murine B cells are recognized by ArtinM via the CRD, and the ArtinM stimulus did not augment the proliferation rate or production of IL-2. However, murine B cell incubation with ArtinM augmented the rate of apoptosis, and this cytotoxic effect of ArtinM was also seen in human B cell-lines sourced from non-Hodgkin's lymphoma Raji cell line. This cytotoxic effect was inhibited by the phosphatase activity of CD45 on Lck, and the protein kinases of the Src family contribute to cell death triggered by ArtinM. (AU)

• Processo FAPESP: 16/04877-2 - Delineamento de novas estratégias terapêuticas, baseadas em reconhecimento de carboidratos, contra a criptococose
• Beneficiário: Thiago Aparecido da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/18538-0 - Bioengenharia de células T e NK através de receptores CAR contra infecções fúngicas invasivas
• Beneficiário: Thiago Aparecido da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/21708-5 - Aplicação de imunomoduladores, via reconhecimento de carboidratos, como agentes terapêuticos: do mecanismo de ação à imunoterapia
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/05867-9 - Efeito da lectina ArtinM em células B e NK
• Beneficiário: Bruno Rafael Barboza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
• Beneficiário: Alexandre Keiji Tashima
• Modalidade de apoio: Auxílio à Pesquisa - Regular