Different virulence genetic context of multidrug-resistant CTX-M- and KPC-producing Klebsiella pneumoniae isolated from cerebrospinal fluid

Autor(es)

Ballaben, Anelise Stella ; Galetti, Renata ; Ferreira, Joseane Cristina ; Paziani, Mario Henrique ; Kress, Marcia Regina von Zeska ; Garcia, Doroti de Oliveira ; da Silva, Paulo ; Doi, Yohei ; Darini, Ana Lucia Costa ; Andrade, Leonardo Neves

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 104, n. 3, p. 5-pg., 2022-08-19.

Resumo

Information regarding resistance and virulence traits of meningitis-causing enterobacteria in hospital environment remains scarce. The aim of this study was to characterize virulence and acquired resistance genes of carbapenem-resistant and/or 3rd to 4th generation cephalosporin-resistant Klebsiella pneumoniae isolated from the cerebrospinal fluid of inpatients. Antimicrobial susceptibility testing was performed by disk diffusion. The string test was performed to identify hypermucoviscous phenotype. Galleria mellonella infection model was used to evaluate the virulence profile of the isolates. Screening for virulence determinants and acquired resistance genes were performed by PCR. The blaCTX-M and/or blaKPC and/or rmtG were detected in all the isolates. Genetic virulence determinants, including mrkD, entB, iroD, fecIRA, uge, wabG, fimH, ureA, ybtS, and clb were detected in the majority of multidrug-resistant K. pneumoniae isolates. One isolate presented hypermucoviscous phenotype, and several isolates showed enhanced virulence in G. mellonella infection model. The combination of the virulence genes found here seems to support not only the known virulence genetic context among nosocomial infections-causing K. pneumoniae but also the role that clb and ybtS may play in K. pneumoniae virulence. (C) 2022 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/11728-0 - Sequenciamento completo de plasmídeos carreando genes de resistência a antibióticos em bactérias de interesse e análise do genoma de linhagens de Pseudomonas aeruginosa produtoras de SPM-1 e de KPC-2 por sequenciamento em larga escala
• Beneficiário: Renata Galetti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23484-9 - Estudo molecular de bactérias gram-negativas produtoras de beta-lactamases isoladas de pacientes com suspeita de meningite na região de Ribeirão Preto
• Beneficiário: Anelise Stella Ballaben
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto