LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

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Teixeira, Franciane Mouradian Emidio ; Oliveira, Luana de Mendonca ; Pietrobon, Anna Julia ; de Salles, Erika Machado ; D'Imperio Lima, Maria Regina ; Viana, Isabelle Freire Tabosa ; Lins, Roberto Dias ; Rigato, Paula Ordonhez ; Marques, Ernesto Torres de Azevedo ; da Silva Duarte, Alberto Jose ; Sato, Maria Notomi

• Tipo de documento: Artigo Científico
• Fonte: VACCINES; v. 10, n. 8, p. 14-pg., 2022-08-01.

Resumo

Neonates have a limited adaptive response of plasma cells, germinal center (GC) B cells, and T follicular helper cells (T-FH). As neonatal vaccination can be an important tool for AIDS prevention, these limitations need to be overcome. Chimeric DNA vaccine encoding p55Gag HIV-1 protein conjugated with lysosomal-associated membrane protein 1 (LAMP-1) has been described as immunogenic in the neonate period. Herein, we investigated the immunologic mechanisms involved in neonatal immunization with a LAMP-1/p55Gag (LAMP/Gag) DNA vaccine in a C57BL/6 mouse background. Neonatal LAMP/Gag vaccination induced strong Gag-specific T-cell response until adulthood and elevated levels of anti-Gag IgG antibodies. We also demonstrated for the first time that the immunogenicity of the neonatal period with LAMP/Gag is due to the induction of high-affinity anti-p24 IgG antibodies and long-term plasma cells. Together with that, there is the generation of early TFH cells and the formation of GC sites with the upregulation of activation-induced cytidine deaminase (AID) enzyme mRNA and protein expression in draining lymph nodes after neonatal LAMP/Gag vaccination. These findings underscore that the LAMP-1 strategy in the chimeric vaccine could be useful to enhance antibody production even in the face of neonatal immaturity, and they contribute to the development of new vaccine approaches for other emerging pathogens at an early stage of life. (AU)

• Processo FAPESP: 19/25119-7 - Interface materno-fetal: imunopatogênese e intervenção vacinal em infecções virais
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/18230-6 - Avaliação da eficácia da vacina de DNA quimérica do Vírus Zika e da proteína de associação à membrana lisossomal em camundongos
• Beneficiário: Franciane Mouradian Emidio Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/18199-9 - Efeito da suplementação materna com o ácido retinoico durante a amamentação na resposta imunológica da prole de camundongos
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular