| Texto completo | |
| Autor(es): |
Cavallone, Italo N.
;
de Lima, Sarah K. S.
;
Oliveira, Karine S.
;
Passero, Luiz Felipe D.
;
Laurenti, Marcia D.
;
Jesus, Jessica Adriana
;
Marinsek, Gabriela P.
;
Chucri, Thais M.
;
Mari, Renata B.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Parasitology; v. 239, p. 10-pg., 2022-07-06. |
| Resumo | |
Visceral leishmaniasis is a neglected tropical disease caused by parasites belonging to the Leishmania genus that infect macrophages in different tissues such as the spleen, liver, lymph nodes, bone marrow, and intestine. Therefore, this study aimed to investigate the integrity of the intestinal tract and the nitrergic (NADPH-dp) and metabolically active (NADH-dp) myenteric neurons of the duodenum of golden hamsters infected with L. (L.) infantum. Therefore, thirty golden hamsters were divided into six groups (n = 5); three of them were infected with 2 x 107 promastigote forms of L. (L.) infantum by intraperitoneal route (Infected Group - IG) and three were inoculated with saline solution (control group - CG). After 30, 60 and 90 days post-infection (DPI) infected animals were euthanized and the liver, spleen and duodenum were collected to analyze tissue parasitism. The duodenum was processed using usual histological techniques to analyze the main changes that occurred during infection and histochemical techniques to phenotype myenteric neurons. Amastigote forms were observed in the spleen, liver, and duodenum during all experimental periods, and tissue parasitism in these organs increased significantly over time. At 30 DPI, reduction in muscle tunic, increase in the total intestinal wall and the number of goblet cells PAS+ was observed. At 60 DPI, an increase in intestinal crypts and intraepithelial lymphocytes was observed, and a reduction in intestinal villi was observed at 90 DPI, along with an increase in crypt size. Regarding neurons, an increase in the density of the NADPH-dp population was observed at 30 DPI, but at 60 and 90 DPI a significant reduction of this population was observed. In general, infection progression was observed to cause significant morphofunctional changes in the duodenum of infected hamsters. (AU) | |
| Processo FAPESP: | 18/24077-6 - Estudos pré-clínicos de tratamentos não invasivos nas leishmanioses |
| Beneficiário: | Luiz Felipe Domingues Passero |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/04869-5 - ALTERAÇÕES HISTOPATOLÓGICAS DO INTESTINO DE HAMSTERS INFECTADOS EXPERIMENTALMENTE POR Leishmania (LEISHMANIA) chagasi |
| Beneficiário: | Sarah Santos de Lima Melchert |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 18/06778-7 - ANÁLISE DA PLASTICIDADE NEURAL MIOENTÉRICA DO DUODENO DE HAMSTERS INFECTADOS COM Leishmania (Leishmania) infantum |
| Beneficiário: | Italo Novais Cavallone |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |