Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects

Autor(es)

Graton, Murilo E. ; Ferreira, Bruno H. S. H. ; Troiano, Jessica A. ; Potje, Simone. R. R. ; Vale, Gabriel. T. T. ; Nakamune, Ana Claudia M. S. ; Tirapelli, Carlos R. ; Miller, Francis. J. J. ; Ximenes, Valdecir. F. F. ; Antoniali, Cristina

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PHYSIOLOGY; v. 13, p. 14-pg., 2022-11-15.

Resumo

Reactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO center dot) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO center dot concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered. (AU)

• Processo FAPESP: 16/22180-9 - Avaliação de mecanismos de modificação pós-translacionais que aumentam a biodisponibilidade do óxido nítrico em vasos de ratas normotensas e espontaneamente hipertensas SHR)ao final da prenhez
• Beneficiário: Cristina Antoniali Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01476-7 - Apocinina: um antioxidante ou inibidor de NAD(P)H oxidase em células vasculares?
• Beneficiário: Bruno Henrique da Silva Homem Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/18436-0 - Estudo dos mecanismos pós-translacionais envolvidos no aumento da biodisponibilidade de óxido nítrico em ratos espontaneamente hipertensos tratados com apocinina
• Beneficiário: Murilo Eduardo Graton
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático