Comparative Genomics of Xylella fastidiosa Explores Candidate Host-Specificity Determinants and Expands the Known Repertoire of Mobile Genetic Elements and Immunity Systems

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Uceda-Campos, Guillermo ; Feitosa-Junior, Oseias R. ; Santiago, Caio R. N. ; Pierry, Paulo M. ; Zaini, Paulo A. ; de Santana, Wesley O. ; Martins, Joaquim ; Barbosa, Deibs ; Digiampietri, Luciano A. ; Setubal, Joao C. ; da Silva, Aline M.

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 10, n. 5, p. 20-pg., 2022-05-01.

Resumo

Xylella fastidiosa causes diseases in many plant species. Originally confined to the Americas, infecting mainly grapevine, citrus, and coffee, X. fastidiosa has spread to several plant species in Europe causing devastating diseases. Many pathogenicity and virulence factors have been identified, which enable the various X. fastidiosa strains to successfully colonize the xylem tissue and cause disease in specific plant hosts, but the mechanisms by which this happens have not been fully elucidated. Here we present thorough comparative analyses of 94 whole-genome sequences of X. fastidiosa strains from diverse plant hosts and geographic regions. Core-genome phylogeny revealed clades with members sharing mostly a geographic region rather than a host plant of origin. Phylogenetic trees for 1605 orthologous CDSs were explored for potential candidates related to host specificity using a score of mapping metrics. However, no candidate host-specificity determinants were strongly supported using this approach. We also show that X. fastidiosa accessory genome is represented by an abundant and heterogeneous mobilome, including a diversity of prophage regions. Our findings provide a better understanding of the diversity of phylogenetically close genomes and expand the knowledge of X. fastidiosa mobile genetic elements and immunity systems. (AU)

• Processo FAPESP: 11/09409-3 - Tecnologia microfluídica para estudo dos mecanismos de adesão de bactérias fitopatogênicas
• Beneficiário: Paulo Adriano Zaini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/11703-4 - Identificação de genes potencialmente associados adaptação, evolução e virulência de Xylella fastidiosa
• Beneficiário: Aline Maria da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/04062-7 - Distribuição, diversidade e dinâmica de sistemas de imunidade antifagos em genomas procarióticos e metagenomas.
• Beneficiário: Guillermo Uceda Campos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/13527-1 - Sequenciamento e análise comparativa do genoma de uma cepa do fitopatógeno Xylella fastidiosa.
• Beneficiário: Paulo Marques Pierry
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/01217-8 - Caracterização da resposta de Citrus sinensis à infecção por Xylella fastidiosa e filogenômica de suas diferentes linhagens.
• Beneficiário: Joaquim Martins Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado