Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling

Autor(es)

Durski, Mariana ; Ravel, Jacques ; Spautz, Ana C. P. G. ; Carvalho, Newton S. ; Silva, Marcia G. ; Marconi, Camila

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 104, n. 1, p. 5-pg., 2022-06-30.

Resumo

Objective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84-0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80-0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV. (c) 2022 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/10403-2 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/16800-3 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22463-5 - Análise proteômica cérvico-vaginal na vaginose bacteriana: associação com a composição bacteriana e resposta ao tratamento com metronidazol
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado