Molecular characterization and sequecing analysis of SARS-CoV-2 genome in Minas Gerais, Brazil

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Ferreira, Giulia Magalhaes ; Claro, Ingra Morales ; Grosche, Victoria Riquena ; Candido, Darlan ; Jose, Diego Pandelo ; Rocha, Esmenia Coelho ; Coletti, Thais de Moura ; Manuli, Erika Regina ; Gaburo Jr, Nelson ; Faria, Nuno Rodrigues ; Sabino, Ester Cerdeira ; de Jesus, Jaqueline Goes ; Jardim, Ana Carolina Gomes

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICALS; v. 80, p. 10-pg., 2022-10-01.

Resumo

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first identified in Wuhan, China, is the causative agent of the coronavirus disease 2019 (COVID-19). Since its first notification in Sa similar to o Paulo state (SP) on 26th February 2020, more than 22,300,000 cases and 619,000 deaths were reported in Brazil. In early pandemic, SARS-CoV-2 spread locally, however, over time, this virus was disseminated to other regions of the country. Herein, we performed genomic sequencing and phylogenetic analysis of SARS-CoV-2 using 20 clinical samples of COVID-19 confirmed cases from 9 cities of Minas Gerais state (MG), in order to evaluate the molecular properties of circulating viral strains in this locality from March to May 2020. Our analyses demonstrated the circulation of B.1 lineage isolates in the investigated locations and nucleotide substitutions were observed into the genomic regions related to important viral structures. Additionally, sequences generated in this study clustered with isolates from SP, suggesting a dissemination route between these two states. Alternatively, monophyletic groups of sequences from MG and other states or country were observed, indicating independent events of virus introduction. These results reinforce the need of genomic surveillance for understand the ongoing spread of emerging viral pathogens. (AU)

• Processo FAPESP: 18/17176-8 - Em tempo real, rápida detecção e sequenciamento de arbovírus no Brasil: Zika, Dengue, Chikungunya e Febre Amarela
• Beneficiário: Ingra Morales Claro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/14389-0 - Centro Conjunto Brasil-Reino Unido para Descoberta, Diagnóstico, Genômica e Epidemiologia de Arbovírus (CADDE)
• Beneficiário: Ester Cerdeira Sabino
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/12000-1 - Modelagem da dispersão espaço-temporal de arbovírus emergentes usando fontes de dados heterogêneas no Estado de São Paulo
• Beneficiário: Jaqueline Goes de Jesus
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado