Genetic polymorphisms in theInterleukins IL1B, IL4,andIL6are associated with concomitant periodontitis and type 2 diabetes mellitus in Brazilian patients

Autor(es)

Cirelli, Thamiris ; Nepomuceno, Rafael ; Rios, Ana Claudia S. ; Orrico, Silvana R. P. ; Cirelli, Joni A. ; Theodoro, Leticia H. ; Barros, Silvana P. ; Scarel-Caminaga, Raquel M.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 55, n. 6, p. 13-pg., 2020-07-09.

Resumo

Objective To assess whether single nucleotide polymorphisms (SNPs) in theIL10, IL1A, IL1B, IL4, TNFA, IL6, OPG, RANK,andRANKLgenes, "classically" related with periodontitis, could be associated with susceptibility to T2DM, and also with both diseases concomitantly. Background There are common pathogenic mechanisms in type 2 diabetes mellitus (T2DM) and periodontitis, but the knowledge of the genetic aspect of this is limited. In patients affected by concomitant T2DM and periodontitis, whose incidence is increasing, there is scarce information regarding the gene-phenotype association, including whether there are genes able to influence both diseases as comorbidities. Methods Periodontal clinical parameters and biochemical profile (Insulin, Fasting Glycemia, HbA1c, Triglycerides, Total Cholesterol, HDL-cholesterol, and LDL-cholesterol) data were obtained from 894 individuals divided into following three groups: Healthy (H; n = 347), Periodontitis (P; n = 348), and Periodontitis + T2DM (P + T2DM; n = 199). DNA from oral epithelial cells was collected for genotyping. Associations between SNPs and pathologies were tested by multiple logistic regression models, adjusting for age, sex, and smoking habits. We also investigated whether there are sex or smoking effects of each SNP in these phenotypes. Results The rs1143634-GA (IL1B)SNP showed significantly less likely to develop P + T2DM for all population and mainly for women (adjusted OR = 0.37, 95% CI = 0.16-0.88), while women carrying the rs224320 CT (IL4)were more susceptible to develop P + T2DM (adjusted OR = 1.81, 95% CI = 1.04-3.15). Men carrying the rs1800795-CC (IL6)genotype were less likely to develop T2DM (adjusted OR = 0.12, 95% CI = 0.02-0.70,P = .01). Conclusions Some SNPs in theIL1B,IL4,andIL6genes demonstrated sex-influenced association with concomitant periodontitis and T2DM, increasing the evidence of a common genetic component between these diseases and contributing with the understanding of their common pathogenic mechanisms. (AU)

• Processo FAPESP: 16/08070-6 - Investigação da suscetibilidade genética à periodontite crônica associada ao Diabetes mellitus tipo 2
• Beneficiário: Thamiris Cirelli
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/13295-1 - Investigação da associação de variações genéticas na suscetibilidade à doença periodontal crônica por meio de OpenArray®
• Beneficiário: Rafael Nepomuceno Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/03753-8 - Investigação de potenciais marcadores genéticos da periodontite crônica associada ou não ao Diabetes Mellitus tipo 2 por meio da suscetibilidade genética e do perfil glicêmico e lipídico do paciente
• Beneficiário: Raquel Mantuaneli Scarel Caminaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/18313-3 - Investigação da susceptibilidade de variações genéticas a Periodontite Crônica por OpenArray®: Bioestatística, Bioinformática e Análise Funcional
• Beneficiário: Rafael Nepomuceno Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado