Hla-C genetic diversity and evolutionary insights in two samples from Brazil and Benin

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Souza, Andreia S. ; Sonon, Paulin ; Paz, Michelle A. ; Tokplonou, Leonidas ; Lima, Thalitta H. A. ; Porto, Iane O. P. ; Andrade, Heloisa S. ; Silva, Nayane dos S. B. ; Veiga-Castelli, Luciana C. ; Oliveira, Maria Luiza G. ; Sadissou, Ibrahim Abiodoun ; Massaro, Juliana Doblas ; Moutairou, Kabirou A. ; Donadi, Eduardo A. ; Massougbodji, Achille ; Garcia, Andre ; Ibikounle, Moudachirou ; Meyer, Diogo ; Sabbagh, Audrey ; Mendes-Junior, Celso T. ; Courtin, David ; Castelli, Erick C.

• Tipo de documento: Artigo Científico
• Fonte: HLA; v. 96, n. 4, p. 19-pg., 2020-08-24.

Resumo

Human leukocyte antigen-C (HLA-C) is a classical HLA class I molecule that binds and presents peptides to cytotoxic T lymphocytes in the cell surface. HLA-C has a dual function because it also interacts with Killer-cell immunoglobulin-like receptors (KIR) receptors expressed in natural killer and T cells, modulating their activity. The structure and diversity of theHLA-Cregulatory regions, as well as the relationship among variants along theHLA-C locus, are poorly addressed, and few population-based studies explored theHLA-Cvariability in the entire gene in different population samples. Here we present a molecular and bioinformatics method to evaluate the entireHLA-Cdiversity, including regulatory sequences. Then, we applied this method to survey theHLA-Cdiversity in two population samples with different demographic histories, one highly admixed from Brazil with major European contribution, and one from Benin with major African contribution. TheHLA-Cpromoter and 3 ' UTR were very polymorphic with the presence of few, but highly divergent haplotypes. These segments also present conserved sequences that are shared among different primate species. Nucleotide diversity was higher in other segments rather than exons 2 and 3, particularly around exon 5 and the second half of the 3 ' UTR region. We detected evidence of balancing selection on the entireHLA-C locusand positive selection in the HLA-C leader peptide, for both populations. HLA-C motifs previously associated with KIR interaction and expression regulation are similar between both populations. Each allele group is associated with specific regulatory sequences, reflecting the high linkage disequilibrium along the entireHLA-C locusin both populations. (AU)

• Processo FAPESP: 17/19223-0 - Análise da variabilidade e estrutura haplotípica dos genes KIR2DL4, KIR3DL2, KIR3DL3, LILRB1(ILT2) e LILRB2 (ILT4) em uma amostra brasileira do estado de São Paulo
• Beneficiário: Erick da Cruz Castelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/17084-2 - Estudo da variabilidade, controle da expressão e história evolutiva dos genes de Classe I do MHC humano
• Beneficiário: Erick da Cruz Castelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular