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| Autor(es): Mostrar menos - |
Ruano, Anna Paula Carreta
;
Costa, Daniela de Jesus Ferreira
;
Braun, Alexcia Camila
;
Torres, Jacqueline Aparecida
;
Barbosa, Pedro Henrique Pereira
;
Abdallah, Emne Ali
;
Barberan, Caroline
;
Silva, Virgilio Souza E.
;
Bovolim, Graziele
;
Buim, Marcilei E. C.
;
Alves, Vanessa da Silva
;
Riechelmann, Rachel P.
;
Chinen, Ludmilla Thome Domingos
Número total de Autores: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PATHOLOGY RESEARCH AND PRACTICE; v. 243, p. 8-pg., 2023-01-31. |
| Resumo | |
Localized anal cancer is mostly represented by squamous cell carcinoma of the anus (SCCA) and is cured in >= 80 % of cases by chemoradiation (CRT). Development of techniques for detection/evaluating circulating tumor cells (CTCs) for diagnosis/ prognosis/response to therapy can change the manner we treat/follow SCCA patients. Objective: to detect CTCs from patients with SCCA and evaluate the presence of HPV virus, p16 expression and markers related to resistance to CRT (RAD23B/ ERCC1/ TYMS) in CTCs at baseline and after CRT. Methods: CTCs were isolated/quantified by ISET (R), protein expressions were analyzed by immunocytochemistry and HPV DNA was detected by chromogenic in situ hybridization. Results: We enrolled 15 patients: median age was 61 (43-73) years, the majority was women (10/15). CTCs were detected in all patients at baseline (median= 0.4 (0.4-3.33) CTCs/mL) and in 8/9 patients, after CRT (median= 2.33 (0-7.0) CTCs/mL). DNA from HPV was found in CTCs in 14/15 patients (93.33 %) at baseline and in 7/9 (77.7 %) after treatment. At a median follow-up of 22.20 (1.45-38.55) months, three patients expressed ERCC1 in CTCs after treatment, with one of them having disease recurrence. Conclusion: We showed that detection of HPV in CTCs from patients with non-metastatic SCCA is feasible and appears to be a sensitive diagnostic method. These results may be clinically useful for better monitoring these patients. However, future larger cohorts may demonstrate whether there is any correlation between the presence of HPV and the expression of screening markers for CRT in SCCA. (AU) | |
| Processo FAPESP: | 15/16952-6 - Identificação de Proteínas Relacionadas à Resistência a Quimioterapia em Células Tumorais Circulantes de Pacientes com Câncer de Cólon Localmente Avançado |
| Beneficiário: | Emne Ali Abdallah |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/01938-9 - Detecção e avaliação molecular de células tumorais circulantes em pacientes com carcinoma renal de células claras. |
| Beneficiário: | Caroline Correia Ghensev Barberan |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 21/04920-3 - Avaliação e caracterização molecular de células tumorais circulantes de pacientes com Câncer de Ovário |
| Beneficiário: | Anna Paula Carreta Ruano |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 19/18100-8 - Análise de marcadores sanguíneos em pacientes com Osteossarcoma, Sarcomas de Partes Moles e Tumor Desmóide |
| Beneficiário: | Alexcia Camila Braun |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/04150-3 - Avaliação da expressão proteica de PD-L1 em células tumorais circulantes (CTCs) de pacientes com câncer de reto |
| Beneficiário: | Pedro Henrique Barbosa Pereira |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |