Mutagenicity, hepatotoxicity, and neurotoxicity of glyphosate and fipronil commercial formulations in Amazon turtles neonates (Podocnemis expansa)

Autor(es)

Mendonca, Juliana dos Santos ; de Almeida, Julio Cesar Neves ; Vieira, Lucelia Goncalves ; Hirano, Liria Queiroz Luz ; Santos, Andre Luiz Quagliatto ; Andrade, Denis Vieira ; Malafaia, Guilherme ; de Oliveira Jr, Robson Jose ; Beletti, Marcelo Emilio

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 898, p. 12-pg., 2023-11-10.

Resumo

Pesticides are considered one of the main causes of the population decline of reptiles worldwide, with freshwater turtles being particularly susceptible to aquatic contamination. In this context, we investigated the potential mutagenic, hepatotoxic, and neurotoxic effects in neonates of Podocnemis expansa exposed to substrate contaminated with different concentrations of glyphosate and/or fipronil during embryonic development. Eggs collected from the natural environment were artificially incubated in sand moistened with pure water, water added with glyphosate Atar 48 & REG; at concentrations of 65 and 6500 & mu;g/L (groups G1 and G2, respectively), water added with fipronil Regent & REG; 800WG at 4 and 400 & mu;g/L (groups F1 and F2, respectively) and, water added with the combination of 65 & mu;g/L glyphosate and 4 & mu;g/L fipronil or with 6500 & mu;g/L glyphosate and 400 & mu;g/L fipronil (groups GF1 and GF2, respectively). For mutagenicity analysis, we evaluated the frequency of micronuclei (MN) and other erythrocyte nuclear abnormalities (ENAs), while for evaluation of hepatotoxicity and neurotoxicity, livers and encephalon were analyzed for histopathological alterations. Exposure to pesticides, alone or in combination, increased the frequency of erythrocyte nuclear abnormalities, particularly blebbed nuclei, moved nuclei, and notched nuclei. Individuals exposed to fipronil exhibited congestion and inflammatory infiltrate in their liver tissue, while, in the encephalon, congestion, and necrosis were present. Our study confirms that the incubation of eggs in substrate polluted with glyphosate and fipronil causes histopathological damage and mutagenic alteration in P. expansa, highlighting the importance of using different biomarkers to evaluate the ecotoxicological effects of these pesticides, especially in oviparous animals. (AU)

• Processo FAPESP: 21/10910-0 - Capacidades e tolerâncias fisiológicas frente a mudanças climáticas: explorando a dimensão funcional em vertebrados ectotérmicos
• Beneficiário: Kênia Cardoso Bícego
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/04190-9 - Fisiologia termal e balanço de água em anfíbios anuros ao longo de um gradiente altitudinal da Floresta Atlântica
• Beneficiário: Denis Otavio Vieira de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05839-2 - Anuros da Floresta Atlântica: Incorporando informações ecofisiológicas na avaliação de padrões de ocupação de habitat, contemporâneos e futuros
• Beneficiário: Denis Otavio Vieira de Andrade
• Modalidade de apoio: Bolsas no Exterior - Pesquisa