Busca avançada
Ano de início
Entree


Citrullination of actin-ligand and nuclear structural proteins, cytoskeleton reorganization and protein redistribution across cellular fractions are early events in ionomycin-induced NETosis

Texto completo
Autor(es):
Reis, Lorenna Rocha ; Junior, Douglas Ricardo Souza ; Tomasin, Rebeka ; Bruni-Cardoso, Alexandre ; Di Mascio, Paolo ; Ronsein, Graziella Eliza
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: REDOX BIOLOGY; v. 64, p. 14-pg., 2023-06-23.
Resumo

Neutrophil extracellular traps (NETs) are web-like structures of DNA coated with cytotoxic proteins and histones released by activated neutrophils through a process called NETosis. NETs release occurs through a sequence of highly organized events leading to chromatin expansion and rupture of nuclear and cellular membranes. In calcium ionophore-induced NETosis, the enzyme peptidylargine deiminase 4 (PAD4) mediates chromatin decondensation through histone citrullination, but the biochemical pathways involved in this process are not fully understood. Here we use live-imaging microscopy and proteomic studies of the neutrophil cellular fractions to investigate the early events in ionomycin-triggered NETosis. We found that before ionomycin-stimulated neutrophils release NETs, profound biochemical changes occur in and around their nucleus, such as, cytoskeleton reorganization, nuclear redistribution of actin-remodeling related proteins, and citrullination of actin-ligand and nuclear structural proteins. Ionomycin-stimulated neutrophils rapidly lose their characteristic polymorphic nucleus, and these changes are promptly communicated to the extracellular environment through the secretion of proteins related to immune response. Therefore, our findings revealed key biochemical mediators in the early process that subsequently culminates with nuclear and cell membranes rupture, and extracellular DNA release. (AU)

Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/00696-3 - Compreendendo composição e função da HDL através de proteômica
Beneficiário:Graziella Eliza Ronsein
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 17/17270-1 - Estudo do efeito da HDL em neutrófilos ativados
Beneficiário:Lorenna Rocha Reis
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 19/25702-4 - Compreendendo os efeitos da lipoproteína de alta densidade em macrófagos humanos
Beneficiário:Douglas Ricardo de Souza Junior
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto