Phenotypic changes in low-density lipoprotein particles as markers of adverse clinical outcomes in COVID-19

Autor(es):Mostrar menos -

Carmo, Helison Rafael P. ; Yoshinaga, Marcos Y. ; Castillo, Alejandro Rosell ; Chaves-Filho, Adriano Britto ; Bonilha, Isabella ; Barreto, Joaquim ; Muraro, Stefanie Primon ; de Souza, Gabriela Fabiano ; Davanzo, Gustavo Gastao ; Perroud Jr, Mauricio W. ; Lukhna, Kishal ; Ntsekhe, Mpiko ; Davidson, Sean ; Velloso, Licio A. ; Nadruz, Wilson ; Carvalho, Luiz Sergio F. ; Sainz-Jaspeado, Miguel ; Farias, Alessandro S. ; Proenca-Modena, Jose Luiz ; Moraes-Vieira, Pedro M. ; Karathanasis, Sotirios K. ; Yellon, Derek ; Miyamoto, Sayuri ; Remaley, Alan T. ; Sposito, Andrei C.

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR GENETICS AND METABOLISM; v. 138, n. 4, p. 8-pg., 2023-03-06.

Resumo

Background and aims: Low-density lipoprotein (LDL) plasma concentration decline is a biomarker for acute inflammatory diseases, including coronavirus disease-2019 (COVID-19). Phenotypic changes in LDL during COVID-19 may be equally related to adverse clinical outcomes.Methods: Individuals hospitalized due to COVID-19 (n = 40) were enrolled. Blood samples were collected on days 0, 2, 4, 6, and 30 (D0, D2, D4, D6, and D30). Oxidized LDL (ox-LDL), and lipoprotein-associated phospholipase A2 (Lp-PLA2) activity were measured. Ina consecutive series of cases (n = 13), LDL was isolated by gradient ultracentrifugation from D0 and D6 and was quantified by lipidomic analysis. Association between clinical outcomes and LDL phenotypic changes was investigated.Results: In the first 30 days, 42.5% of participants died due to Covid-19. The serum ox-LDL increased from D0 to D6 (p < 0.005) and decreased at D30. Moreover, individuals who had an ox-LDL increase from D0 to D6 to over the 90th percentile died. The plasma Lp-PLA2 activity also increased progressively from D0 to D30 (p < 0.005), and the change from D0 to D6 in Lp-PLA2 and ox-LDL were positively correlated (r = 0.65, p < 0.0001). An exploratory untargeted lipidomic analysis uncovered 308 individual lipids in isolated LDL particles. Paired-test analysis from D0 and D6 revealed higher concentrations of 32 lipid species during disease progression, mainly represented by lysophosphatidyl choline and phosphatidylinositol. In addition, 69 lipid species were exclusively modulated in the LDL particles from non-survivors as compared to survivors.Conclusions: Phenotypic changes in LDL particles are associated with disease progression and adverse clinical outcomes in COVID-19 patients and could serve as a potential prognostic biomarker.(c) 2023 Published by Elsevier Inc. (AU)

• Processo FAPESP: 20/04558-0 - Caracterização de fatores de risco intrínsecos e o desenvolvimento de novas alternativas de diagnóstico e tratamento para COVID-19
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/12649-5 - Estudo multicêntrico, prospectivo, randomizado, duplo-cego e controlado por placebo para avaliar o efeito da diacereína para prevenção de morte ou disfunção de múltiplos órgãos em pacientes com SDRA secundário ao COVID-19
• Beneficiário: Alejandro Rosell Castillo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/00194-8 - Patogênese e neurovirulência de vírus emergentes no Brasil
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 18/17792-0 - HDL-S1P contra os danos da piroptose após Infarto Agudo do Miocárdio
• Beneficiário: Helison Rafael Pereira Do Carmo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado