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Brazilian red propolis extract free and encapsulated into polymeric nanoparticles against ovarian cancer: formulation, characterisation and biological assays in 2D and 3D models

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Autor(es):
Justino, Isabela A. ; Marincek, Andreia ; Ferreira, Iasmin R. S. ; Amaral, Robson L. F. ; Fontanezi, Bianca B. ; Aldana-Mejia, Jennyfer A. ; Bastos, Jairo K. ; Marcato, Priscyla D.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Journal of Pharmacy and Pharmacology; v. 75, n. 6, p. 13-pg., 2023-04-13.
Resumo

Cancer incidence worldwide is alarming and among the cancers that affect women ovarian cancer is the most fatal. Many side effects are associated with conventional therapies and none of them are completely effective, so the development of new treatments is necessary. Brazilian red propolis extract is a natural product with complex composition and great potential for cancer treatment. However, its clinical application is harmed due to unfavourable physicochemical characteristics. To enable its application encapsulation in nanoparticles can be used. Objectives: The aims of this work were to develop polymeric nanoparticles with Brazilian red propolis extract and compare their action with the free extract against ovarian cancer cells. Methods: Box Behnken design was used and nanoparticles were characterised using the techniques dynamic light scattering, nanoparticle tracking analysis, transmission electron microscopy, differential scanning calorimetry and encapsulation efficiency. Activity against OVCAR-3 was also tested on 2D and 3D models. Key findings: Nanoparticles' sizes were similar to 200 nm with monomodal size distribution, negative zeta potential, spherical shape and with extract molecularly dispersed. Encapsulation efficiency was above 97% for the biomarkers chosen. Nanoparticles had greater efficacy in comparison with free propolis in OVCAR-3. Conclusions: So far, the nanoparticles here described have the potential to be a chemotherapy treatment in the future. (AU)

Processo FAPESP: 18/13465-5 - Desenvolvimento de nanopartículas poliméricas contendo própolis vermelha brasileira e avaliação antitumoral in vitro e in vivo em cânceres urológicos
Beneficiário:Priscyla Daniely Marcato Gaspari
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/16921-6 - Desenvolvimento de carreadores lipídicos nanoestruturados como sistema de carreamento de curcumina e avaliação biológica in vitro em células de câncer de bexiga
Beneficiário:Jacqueline Campos Silva
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
Beneficiário:Maria Vitória Lopes Badra Bentley
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
Beneficiário:Jairo Kenupp Bastos
Modalidade de apoio: Auxílio à Pesquisa - Temático