Citrus flavanone metabolites significantly modulate global proteomic profile in pancreatic beta-cells under high-glucose-induced metabolic stress

Autor(es)

Fraga, Layanne Nascimento ; Milenkovic, Dragan ; Anacleto, Sara Lima ; Salemi, Michelle ; Lajolo, Franco Maria ; Hassimotto, Neuza Mariko Aymoto

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1871, n. 3, p. 21-pg., 2023-02-02.

Resumo

Hesperidin and narirutin are the major citrus flavanones. Several studies have associated these compounds with pancreatic beta-cell survival through their capacity to reduce oxidative stress, inflammation, and inhibit apoptosis. However, the molecular mechanisms of action of flavanones in pancreatic beta-cells under high-glycemic stress is still largely unknown. Therefore, this study aimed to decipher molecular mechanisms of flavanone metabolites in pancreatic beta-cells treated with high glucose concentration using untargeted shotgun proteomics. We identified 569 proteins differentially expressed in cells exposed to hesperetin 7-glucuronide (H7G) and 265 in cells exposed to 3-(4'-hydroxyphenyl) propanoic acid (PA). Comparison of global proteomic profiles suggest that these metabolites could counteract changes in protein expression induced by high glucose stress. The bioinformatic analyses suggested that H7G and PA modulated the expression of proteins involved in cell adhesion, cell signaling, metabolism, inflammation, and protein processing in endoplasmic reticulum (ER) pathways. Taken together, this study suggests that H7G and PA can modulate the expression of proteins that may prevent dysfunction of pancreatic beta-cells under stress induced by high glucose. (AU)

• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 18/26584-2 - Efeito de flavanonas de citrus em células Min6 submetidas ao estresse oxidativo: abordagem proteômica
• Beneficiário: Sara Lima Anacleto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 18/03965-0 - Flavanonas de Citrus- mecanismos de proteção de células beta-pancreáticas submetidas ao estresse oxidativo
• Beneficiário: Sara Lima Anacleto
• Modalidade de apoio: Bolsas no Brasil - Mestrado