| Texto completo | |
| Autor(es): |
Acunha, Tanize
;
Rocha, Bruno Alves
;
Nardini, Viviani
;
Barbosa Jr, Fernando
;
Faccioli, Lucia Helena
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 86, n. 9, p. 13-pg., 2023-03-11. |
| Resumo | |
Due to the high prevalence and clinical relevance, scorpionism is a critical public health issue in several Brazilian regions. Tityus serrulatus, commonly known as the Brazilian yellow scorpion, is the most venomous genus found in Brazilian fauna and associated with severe clinical manifestations such as localized pain, hypertension, sweating, tachycardia and complex hyperinflammatory responses. In general, T. serrulatus venom contains a complex mixture of active compounds, including proteins, peptides, and amino acids. Although knowledge of the protein fractions of scorpion venom is available, venom lipid components are not yet comprehensively known. The aim of the present study was to determine and characterize the lipid constituents/profile of the T. serratus venom utilizing liquid chromatography coupled with high-resolution mass spectrometry. Lipid species (164 in total) belonging to 3 different lipid categories, glycerophospholipids, sphingolipids, and glycerolipids, were identified. A further search on MetaCore/MetaDrug platform, which is based upon a manually curated database of molecular interactions, molecular pathways, gene-disease associations, chemical metabolism, and toxicity information, exhibited several metabolic pathways for 24 of previously identified lipid species, including activation of nuclear factor kappa B and oxidative stress pathways. Further several bioactive compounds, such as plasmalogens, lyso-platelet-activating factors, and sphingomyelins, associated with systemic responses triggered by T. serrulatus envenomation were detected. Finally, lipidomic data presented provide advanced and valuable information to better comprehend the mechanisms underlying the complex pathophysiology induced by T. serrulatus envenomation. (AU) | |
| Processo FAPESP: | 15/00658-1 - Novos aspectos funcionais dos eicosanóides |
| Beneficiário: | Lúcia Helena Faccioli |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 21/03633-0 - Desenvolvimento e aplicação de métodos analíticos simples e rápidos para a determinação de desreguladores endócrinos e biomarcadores de estresse oxidativo em amostras biológicas para avaliação da exposição de crianças e gestantes |
| Beneficiário: | Bruno Alves Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua |
| Beneficiário: | Fernando Barbosa Júnior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 14/07125-6 - Novos aspectos funcionais dos eicosanóides |
| Beneficiário: | Lúcia Helena Faccioli |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/07979-1 - Metabolômica do expossoma por espectrometria de massas |
| Beneficiário: | Bruno Alves Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 18/25704-4 - Análise lipidômica empregando espectrometria de mobilidade iônica diferencial (DMS) para determinação de perfil de lipídeos em peçonhas de escorpiões e serpentes |
| Beneficiário: | Tanize dos Santos Acunha |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |