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Nanoemulsions Based on Sunflower and Rosehip Oils: The Impact of Natural and Synthetic Stabilizers on Skin Penetration and an Ex Vivo Wound Healing Model

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Autor(es):
Pereira Oliveira, Cynthia Nara ; Nani Leite, Marcel ; de Paula, Natalia Aparecida ; Araujo Martins, Yugo ; Figueiredo, Sonia Aparecida ; Cipriani Frade, Marco Andrey ; Lopez, Renata Fonseca Vianna
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: PHARMACEUTICS; v. 15, n. 3, p. 24-pg., 2023-03-01.
Resumo

Vegetable oils offer excellent biological properties, but their high lipophilicity limits their bioavailability. This work aimed to develop nanoemulsions based on sunflower and rosehip oils and to evaluate their wound-healing activity. The influence of phospholipids of plant origin on nanoemulsions' characteristics was investigated. A nanoemulsion prepared with a mixture of phospholipids and synthetic emulsifiers (Nano-1) was compared with another prepared only with phospholipids (Nano-2). The healing activity was evaluated in wounds induced in human organotypic skin explant culture (hOSEC) based on histological and immunohistochemical analysis. The hOSEC wound model was validated, showing that high nanoparticle concentration in the wound bed interferes with cell mobility and the ability to respond to the treatment. Nanoemulsions were 130 to 370 nm, with a concentration of 10(13) particles/mL, and a low potential to induce inflammatory processes. Nano-2 was three times larger than Nano-1 but less cytotoxic and could target the oils to the epidermis. Nano-1 permeated intact skin to the dermis and showed a more prominent healing effect than Nano-2 in the hOSEC wound model. Changes in the lipid nanoemulsion stabilizers impacted the cutaneous and cellular penetration of the oils, cytotoxicity, and healing kinetics, resulting in versatile delivery systems. (AU)

Processo FAPESP: 14/22451-7 - Sistemas de liberação sustentada e direcionada de fármacos para o tecido epitelial
Beneficiário:Renata Fonseca Vianna Lopez
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/16437-7 - Avaliação da viabilidade do modelo de pele humana ex vivo (hOSEC) e sua aplicação em estudos de eficácia de protetores solares a longo prazo no fotoenvelhecimento
Beneficiário:Marco Andrey Cipriani Frade
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
Beneficiário:Maria Vitória Lopes Badra Bentley
Modalidade de apoio: Auxílio à Pesquisa - Temático