Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense

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Barioni, Eric Diego ; Santin, Jose Roberto ; Machado, Isabel Daufenback ; de Paula Rodrigues, Stephen Fernandes ; Ferraz-de-Paula, Viviane ; Wagner, Theodoro Marcel ; Cogliati, Bruno ; dos Santos, Matheus Correa ; Machado, Marina da Silva ; de Andrade, Sergio Faloni ; Niero, Rivaldo ; Poliselli Farsky, Sandra Helena

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2013, p. 12-pg., 2013-01-01.

Resumo

Achyrocline satureioides (Lam.) D. C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate-(PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 andCINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, beta 2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity. (AU)

• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/15115-2 - CARACTERIZAÇÃO DAS AÇÕES DO EXTRATO DA INFLORESCÊNCIA DA Achyrocline satureoides SOBRE A FUNÇÃO DE NEUTRÓFILOS NA INFLAMAÇÃO.
• Beneficiário: Eric Diego Barioni
• Modalidade de apoio: Bolsas no Brasil - Mestrado