DNA Methylation Levels of Melanoma Risk Genes Are Associated with Clinical Characteristics of Melanoma Patients

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de Araujo, Erica S. S. ; Pramio, Dimitrius T. ; Kashiwabara, Andre Y. ; Pennacchi, Paula C. ; Maria-Engler, Silvya S. ; Achatz, Maria I. ; Campos, Antonio H. J. F. M. ; Duprat, Joao P. ; Rosenberg, Carla ; Carraro, Dirce M. ; Krepischi, Ana C. V.

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2015, p. 8-pg., 2015-01-01.

Resumo

In melanoma development, oncogenic process is mediated by genetic and epigenetic mutations, and few studies have so far explored the role of DNA methylation either as predisposition factor or biomarker. We tested patient samples for germline CDKN2A methylation status and found no evidence of inactivation by promoter hypermethylation. We have also investigated the association of clinical characteristics of samples with the DNA methylation pattern of twelve genes relevant for melanomagenesis. Five genes (BAP1, MGMT, MITF, PALB2, and POT1) presented statistical association between blood DNA methylation levels and either CDKN2A-mutation status, number of lesions, or Breslow thickness. In tumors, five genes (KIT, MGMT, MITF, TERT, and TNF) exhibited methylation levels significantly different between tumor groups including acral compared to nonacral melanomas and matched primary lesions and metastases. Our data pinpoint that the methylation level of eight melanoma-associated genes could potentially represent markers for this disease both in peripheral blood and in tumor samples. (AU)

• Processo FAPESP: 12/13963-9 - Perfil global de metilação do genoma na síndrome do melanoma familial
• Beneficiário: Érica Sara Souza de Araújo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/10785-5 - Investigação do perfil mutacional em melanomas cutâneos e impacto no padrão global de metilação de DNA
• Beneficiário: Dimitrius Tansini Pramio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/07480-8 - Fatores genéticos e epigenéticos na etiologia do melanoma cutâneo
• Beneficiário: Ana Cristina Victorino Krepischi
• Modalidade de apoio: Auxílio à Pesquisa - Regular