Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5 '-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway

Autor(es)

Torini, Juliana Roberta ; Brandao-Neto, Jose ; DeMarco, Ricardo ; Pereira, Humberto D'Muniz

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 10, n. 12, p. 25-pg., 2016-12-01.

Resumo

Schistosoma mansoni do not have de novo purine pathways and rely on purine salvage for their purine supply. It has been demonstrated that, unlike humans, the S. mansoni is able to produce adenine directly from adenosine, although the enzyme responsible for this activity was unknown. In the present work we show that S. mansoni 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, E.C. 2.4.2.28) is capable of use adenosine as a substrate to the production of adenine. Through kinetics assays, we show that the Schistosoma mansoni MTAP (SmMTAP), unlike the mammalian MTAP, uses adenosine substrate with the same efficiency as MTA phosphorolysis, which suggests that this enzyme is part of the purine pathway salvage in S. mansoni and could be a promising target for anti-schistosoma therapies. Here, we present 13 SmMTAP structures from the wild type (WT), including three single and one double mutant, and generate a solid structural framework for structure description. These crystal structures of SmMTAP reveal that the active site contains three substitutions within and near the active site when compared to it mammalian counterpart, thus opening up the possibility of developing specific inhibitors to the parasite MTAP. The structural and kinetic data for 5 substrates reveal the structural basis for this interaction, providing substract for inteligent design of new compounds for block this enzyme activity. (AU)

• Processo FAPESP: 12/10213-9 - Determinação estrutural e funcional das enzimas Metiltioadenosina Fosforilase, Purina Nucleosídeo Fosforilase isoforma 2 e das enzimas participantes da produção de GTP da via de salvação de purinas em Schistosoma mansoni.
• Beneficiário: Juliana Roberta Torini de Souza Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/14223-9 - Estudos estruturais e cinéticos das enzimas da via de salvação de purina de Schistosoma mansoni
• Beneficiário: Humberto D'Muniz Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular