Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Autor(es)

Bella, Leonardo M. ; Fieri, Isis ; Tessaro, Fernando H. G. ; Nolasco, Eduardo L. ; Nunes, Fernanda P. B. ; Ferreira, Sabrina S. ; Azevedo, Carolina B. ; Martins, Joilson O.

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2017, p. 10-pg., 2017-01-01.

Resumo

Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60mg/kg i.v., 10 days) and non diabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice. (AU)

• Processo FAPESP: 13/20904-1 - Estudo da expressão do receptor de Vitamina D em macrófagos alveolar e peritoneal em camundongos sadios e diabéticos
• Beneficiário: Isis Fieri
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/05100-4 - A vitamina D modula parâmetros hematológicos e a migração celular para as cavidades peritoneal e pulmonar em ratos tornados diabéticos por aloxana
• Beneficiário: Joilson de Oliveira Martins
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 12/23998-4 - Influência da vitamina D na expressão de receptores (VDR e TLR) em macrófagos no modelo de Diabetes Mellitus Experimental
• Beneficiário: Leonardo Mendes Bella
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/02272-0 - Efeito da insulina na inflamação pulmonar secundária a sepse, na imunidade inata, na ativação do seu gene (BGK) e seus receptores (IR-A e IR-B)
• Beneficiário: Joilson de Oliveira Martins
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/05214-1 - Investigando o papel da insulina em diferentes infecções em animais diabéticos e sadios
• Beneficiário: Joilson de Oliveira Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular