| Texto completo | |
| Autor(es): |
da Silva, Ronivaldo Rodrigues
;
Souto, Tatiane Beltramini
;
da Rosa, Nathalia Gonsales
;
Goncalves de Oliveira, Lilian Caroline
;
Juliano, Maria Aparecida
;
Juliano, Luiz
;
Rosa, Jose C.
;
Cabral, Hamilton
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 50, n. 3, p. 8-pg., 2019-10-26. |
| Resumo | |
Traditionally, chymosin has been used for milk-clotting, but this naturally occurring enzyme is in short supply and its use has raised religious and ethical concerns. Because milk-clotting peptidases are a promising substitute for chymosin in cheese preparation, there is a need to find and test the specificity of these enzymes. Here, we evaluated the milk-clotting properties of an aspartic peptidase secreted by Rhizopus microsporus. The molecular mass of this enzyme was estimated at 36 kDa and Pepstatin A was determined to be an inhibitor. Optimal activity occurred at a pH of 5.5 and a temperature range of 50-60 degrees C, but the peptidase was stable in the pH range of 4-7 and a temperature as low as 45 degrees C. Proteolytic activity was significantly reduced in the presence of Cu2+ and Al3+. When enzyme substrates based on FRET were used, this peptidase exhibited the highest catalytic efficiency for Abz-KNRSSKQ-EDDnp (4,644 +/- 155 mM(-1).s(-1)), Abz-KLRSSNQ-EDDnp (3,514 +/- 130 mM(-1).s(-1)), and Abz-KLRQSKQ-EDDnp (3,068 +/- 386 mM(-1).s(-1)). This study presents a promising peptidase for use in cheese making, due to its high stability in the presence of Ca2+ and broad pH range of 4-7, in addition to its ability to efficiently clot milk. (AU) | |
| Processo FAPESP: | 11/06986-0 - Determinação da especificidade de peptidases isoladas de fungos usando peptídeos FRET como substratos |
| Beneficiário: | Hamilton Cabral |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/24703-8 - Análises proteômicas de fungos filamentosos meso e termofílicos expostos aos fatores físico e químico |
| Beneficiário: | Hamilton Cabral |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |