Long-term increase of insulin secretion in mice subjected to pregnancy and lactation

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Vicente, Julia Modesto ; Santos-Silva, Junia Carolina ; Teixeira, Caio Jordao ; de Souza, Dailson Nogueira ; Vettorazzi, Jean Franciesco ; Furtuoso, Fabiola Sales ; Adabo, Isabel Gouveia ; Sato, Fabio Takeo ; Ramirez Vinolo, Marco Aurelio ; Carneiro, Everardo Magalhaes ; Bordin, Silvana ; Anhe, Gabriel Forato

• Tipo de documento: Artigo Científico
• Fonte: ENDOCRINE CONNECTIONS; v. 9, n. 4, p. 10-pg., 2020-04-01.

Resumo

Purpose: Observational studies show that longer breastfeeding periods reduce maternal risk of type 2 diabetes mellitus. However, it is currently unknown if the long-term benefits of breastfeeding for maternal glucose homeostasis are linked to changes in the endocrine pancreas. Methods: We presently evaluated functional, morphological and molecular aspects of the endocrine pancreas of mice subjected to two sequential cycles of pregnancy and lactation (L21). Age-matched mice not allowed to breastfeed (LO) and virgin mice were used as controls. Results: L21 mice exhibited increased tolerance and increased glucose-stimulated insulin secretion (GSIS) by isolated islets. Pancreatic islets of L21 mice did not present evident morphological changes to justify the increased GSIS. On the other hand, islets of L21 mice exhibited a reduction in Cavb3 and Kir6.2 expression with concordant increased intracellular Ca2+ levels after challenge with glucose. Conclusion: Altogether, the present findings show the breastfeeding exerts long-term benefits for maternal endocrine pancreas by increasing intracellular Ca2+ levels and GSIS. (AU)

• Processo FAPESP: 15/12680-1 - Estudo dos impactos em longo prazo da duração da lactação no metabolismo energético materno: participação de microRNAs hepáticos
• Beneficiário: Gabriel Forato Anhê
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/11138-4 - Investigação do efeito da gestação seguida de lactação na modulação de miRNAs de ilhotas pancreáticas maternas
• Beneficiário: Julia Modesto Vicente
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica