Inhibition of alpha v beta 3 integrin impairs adhesion and uptake of tumor-derived small extracellular vesicles

Autor(es)

Altei, Wanessa F. ; Pachane, Bianca C. ; dos Santos, Patty K. ; Ribeiro, Ligia N. M. ; Sung, Bong Hwan ; Weaver, Alissa M. ; Selistre-de-Araujo, Heloisa S.

• Tipo de documento: Artigo Científico
• Fonte: CELL COMMUNICATION AND SIGNALING; v. 18, n. 1, p. 15-pg., 2020-09-25.

Resumo

Background; Extracellular vesicles (EVs) are lipid-bound particles that are naturally released from cells and mediate cell-cell communication. Integrin adhesion receptors are enriched in small EVs (SEVs) and SEV-carried integrins have been shown to promote cancer cell migration and to mediate organ-specific metastasis; however, how integrins mediate these effects is not entirely clear and could represent a combination of EV binding to extracellular matrix and cells. Methods: To probe integrin role in EVs binding and uptake, we employed a disintegrin inhibitor (DisBa-01) of integrin binding with specificity for alpha v beta 3 integrin. EVs were purified from MDA-MB-231 cells conditioned media by serial centrifugation method. Isolated EVs were characterized by different techniques and further employed in adhesion, uptake and co-culture experiments. Results: We find that SEVs secreted from MDA-MB-231 breast cancer cells carry alpha v beta 3 integrin and bind directly to fibronectin-coated plates, which is inhibited by DisBa-01. SEV coating on tissue culture plates also induces adhesion of MDA-MB-231 cells, which is inhibited by DisBa-01 treatment. Analysis of EV uptake and interchange between cells reveals that the amount of CD63-positive EVs delivered from malignant MDA-MB-231 breast cells to non-malignant MCF10A breast epithelial cells is reduced by DisBa-01 treatment. Inhibition of alpha v beta 3 integrin decreases CD63 expression in cancer cells suggesting an effect on SEV content. Conclusion: In summary, our findings demonstrate for the first time a key role of alpha v beta 3 integrin in cell-cell communication through SEVs. (AU)

• Processo FAPESP: 14/18747-8 - Exossomos de células tumorais de mama e seu papel na adesão celular durante o processo de metástase: estudos in vitro
• Beneficiário: Wanessa Fernanda Altei
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/22539-7 - O papel das integrinas na ligação dos exossomos à matriz extracelular e sua contribuição para a progressão tumoral
• Beneficiário: Wanessa Fernanda Altei
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado