Increased Reactive Oxygen Species and Tumor Necrosis Factor-Alpha Production by Monocytes are Associated with Elevated Levels of Uric Acid in Pre-Eclamptic Women

Autor(es)

Peracoli, Maria T. S. ^[1] ; Bannwart, Camila F. ^[2] ; Cristofalo, Renata ^[1] ; Medeiros Borges, Vera T. ^[2] ; Araujo Costa, Roberto A. ^[2] ; Witkin, Steven S. ^[3] ; Peracoli, Jose C. ^[2]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Dept Microbiol & Immunol, Inst Biociences, Sao Paulo - Brazil

^[2] UNESP Sao Paulo State Univ, Dept Obstet & Ginecol, Botucatu Med Sch, Sao Paulo - Brazil

^[3] Cornell Univ, Div Immunol & Infect Dis, Weill Med Coll, Dept Obstet & Gynecol, New York, NY 10021 - USA

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Reproductive Immunology; v. 66, n. 6, p. 460-467, DEC 2011.
• Citações Web of Science: 28

Resumo

Problem To evaluate associations between hyperuricemia and increases in production of reactive oxygen species (ROS) and tumor necrosis factor alpha (TNF-alpha) in pre-eclamptic pregnancies. Method of study This study investigated serum uric acid levels, monocyte production of TNF-alpha, superoxide anion (O(2)(-)) and hydrogen peroxide (H(2)O(2)), as well as superoxide dismutase (SOD) and catalase (CAT) activities in erythrocytes from 30 women with pre-eclampsia (PE) compared with 30 normotensive (NT) pregnant women in the last trimester of pregnancy. Results Serum uric acid levels (6.1 versus 2.8 mg/dL) as well as endogenous O(2)(-) (2.2 versus 1.6 nm), H(2)O(2) (1.8 versus 1.4 nm) and TNF-alpha (91.6 versus 40.4 pg/mL) released from monocytes were significantly higher in the pre-eclamptic group than in the NT group (P < 0.05). SOD activity in erythrocytes was also significantly elevated in the PE group (5969.2 versus 4834.7 U/g Hb). No significant difference between groups was observed in relation to CAT activity. Conclusions Elevated serum uric acid levels are correlated with higher O(2)(-) and TNF-alpha production by monocytes in women with PE. This may contribute to the enhanced oxidative and inflammatory state characteristic of this disorder. (AU)

• Processo FAPESP: 06/07095-3 - Efeito modulador da silimarina (Silybum marianum) no mecanismo fisiopatológico da pré-eclâmpsia
• Beneficiário: Jose Carlos Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular