| Texto completo | |
| Autor(es): |
de Mendonca, Mariana
;
Bolin, Anaysa Paola
;
Oliveira, Nayara Preste de
;
Real, Caroline Cristiano
;
Hu, Xiaoyun
;
Huang, Zhan-Peng
;
Wang, Da-Zhi
;
Rodrigues, Alice Cristina
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE; v. 1870, n. 4, p. 15-pg., 2024-02-15. |
| Resumo | |
During inguinal adipose tissue (iWAT) ontogenesis, beige adipocytes spontaneously appear between postnatal 10 (P10) and P20 and their ablation impairs iWAT browning capacity in adulthood. Since maternal obesity has deleterious effects on offspring iWAT function, we aimed to investigate its effect in spontaneous iWAT browning in offspring. Female C57BL/6 J mice were fed a control or obesogenic diet six weeks before mating. Male and female offspring were euthanized at P10 and P20 or weaned at P21 and fed chow diet until P60. At P50, mice were treated with saline or CL316,243, a beta 3-adrenoceptor agonist, for ten days. Maternal obesity induced insulin resistance at P60, and CL316,243 treatment effectively restored insulin sensitivity in male but not female offspring. This discrepancy occurred due to female offspring severe browning impairment. During development, the spontaneous iWAT browning and sympathetic nerve branching at P20 were severely impaired in female obese dam's offspring but occurred normally in males. Additionally, maternal obesity increased miR-22 expression in the iWAT of male and female offspring during development. ER alpha, a target and regulator of miR22, was concomitantly upregulated in the male's iWAT. Next, we evaluated miR-22 knockout (KO) offspring at P10 and P20. The miR-22 deficiency does not affect spontaneous iWAT browning in females and, surprisingly, anticipates iWAT browning in males. In conclusion, maternal obesity impairs functional iWAT development in the offspring in a sex-specific way that seems to be driven by miR-22 levels and ER alpha signaling. This impacts adult browning capacity and glucose homeostasis, especially in female offspring. (AU) | |
| Processo FAPESP: | 18/07087-8 - O papel dos microRNAs nas alterações metabólicas promovidas pela obesidade induzida por dieta ou por obesidade materna |
| Beneficiário: | Alice Cristina Rodrigues |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/08049-2 - MicroRNAs circulantes como mediadores das adaptações metabólicas promovidas pelo exercício aeróbio na obesidade |
| Beneficiário: | Alice Cristina Rodrigues |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |