hsa-miR-582-3p in umbilical cord blood is negatively associated with maternal exposure to childhood maltreatment

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Micali, Danilo ; Rosa, Joice Santos ; Ota, Vanessa Kiyomi ; Mercuri, Rafael Luiz Vieira ; Spindola, Leticia Maria ; Bugiga, Amanda Victoria Gomes ; Kajitani, Gustavo Satoru ; Milani, Ana Carolina Coelho ; Salmeron, Camilla ; Silva, Ivaldo ; Asprino, Paula Fontes ; Galante, Pedro A. F. ; Duarte, Cristiane Seixas ; Posner, Jonathan ; Jackowski, Andrea Parolin ; Belangero, Sintia Iole

• Tipo de documento: Artigo Científico
• Fonte: Epigenomics; v. N/A, p. 14-pg., 2024-10-09.

Resumo

Aim: Childhood maltreatment (CM) may affect not only directly exposed individuals but also their offspring. However, the underlying biological mechanisms remain unclear. microRNAs (miRNAs) may play a regulatory role in this process. This study investigates the relationship between maternal exposure to CM and miRNA expression in maternal and perinatal tissues. Methods: We enrolled 43 pregnant women and assessed their CM exposure. We collected maternal blood, cord blood and placental tissue samples during childbirth and performed miRNA profiling using next generation sequencing. Results: Maternal CM was inversely associated with hsa-miR-582-3p levels in cord blood. Pathway analysis revealed that this miRNA regulates genes involved in intrauterine development. Conclusion: Our findings highlight the potential impact of maternal CM exposure on offspring epigenetic mechanisms. PLAIN LANGUAGE SUMMARY Child maltreatment (CM) includes physical, sexual and emotional abuse, as well as physical and emotional neglect. CM not only harms those directly exposed but can also negatively impact their offspring. However, the biological reasons behind this are not well understood. To explore this further, our study investigates how CM affects the biology of pregnant women and their newborns through changes in small regulatory molecules called microRNAs (miRNAs). We recruited 43 pregnant women and assessed their exposure to CM. During childbirth, we collected blood samples from the mothers, blood from the umbilical cord and placental samples. We then analyzed the levels of miRNAs in these samples using advanced sequencing technology. We observed that more severe maternal exposure to CM was associated with lower levels of a miRNA named hsa-miR-582-3p in umbilical cord blood. This miRNA regulates genes involved in fetal development in utero and has been linked to spontaneous preterm birth. It may also influence immunologic and stress-related processes. Thus, newborns of mothers who had been exposed to CM may be more vulnerable to adverse effects on their brain development and overall health. Despite our small sample size, our study highlights the importance of addressing CM as an intergenerational concern and provides new insights into the biological mechanisms through which maternal CM can affect offspring. (AU)

• Processo FAPESP: 19/21612-0 - Adversidades materna, inflamação e neurodesenvolvimento: como os processos intergeracionais perpetuam as desigualdades em um ambiente com poucos recursos
• Beneficiário: Andrea Parolin Jackowski
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/15579-8 - Retroelementos: uma força motriz criando novidades genéticas no genoma humano e de camundongos
• Beneficiário: Pedro Alexandre Favoretto Galante
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 20/02413-4 - Retrocópias intragênicas como fonte de novos domínios proteicos em humanos
• Beneficiário: Rafael Luiz Vieira Mercuri
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto