High levels of multidrug- resistant isolates of genetically similar Salmonella 1,4, [5],12:I:- from Brazil between 1983 and 2020

Autor(es)

Pereira, Giovana do Nascimento ; Seribelli, Amanda Aparecida ; Campioni, Fabio ; Gomes, Carolina Nogueira ; Tiba-Casas, Monique Ribeiro ; Medeiros, Marta Ines Cazentini ; Rodrigues, Dalia dos Prazeres ; Falcao, Juliana Pfrimer

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Microbiology; v. 73, n. 2, p. 15-pg., 2024-01-01.

Resumo

Introduction. Salmonella 1,4, [5],12:i:- strains with different antimicrobial resistance profiles have been associated with foodborne disease outbreaks in several countries. In Brazil, S. 1,4, [5],12:i:- was identified as one of the most prevalent serovars in S & atilde;o Paulo State during 2004-2020. Gap Statement. However, few studies have characterized this serovar in Brazil. Aim. This study aimed to determine the antimicrobial resistance profiles of S. 1,4, [5],12:i:- strains isolated from different sources in Southeast Brazil and compare their genetic diversity. Methodology. We analysed 113 S. 1,4, [5],12:i:- strains isolated from humans (n=99), animals (n=7), food (n=5) and the environment (n=2) between 1983 and 2020. Susceptibility testing against 13 antimicrobials was performed using the disc diffusion method for all the strains. Plasmid resistance genes and mutations in the quinolone resistance- determining regions were identified in phenotypically fluoroquinolone- resistant strains. Molecular typing was performed using enterobacterial repetitive intergenic consensus PCR (ERIC- PCR) for all strains and multilocus sequence typing (MLST) for 40 selected strains. Results. Of the 113 strains, 54.87 % were resistant to at least one antimicrobial. The highest resistance rates were observed against ampicillin (51.33 %), nalidixic acid (39.82 %) and tetracycline (38.05 %). Additionally, 39 (34.51 %) strains were classified as multidrug- resistant (MDR). Nine fluoroquinolone- resistant strains exhibited the gyrA mutation (Ser96 -> Tyr96) and contained the qnrB gene. The 113 strains were grouped into two clusters using ERIC- PCR, and most of strains were present in one cluster, with a genetic similarity of >= 80 %. Finally, 40 strains were typed as ST19 using MLST. Conclusion. The prevalence of MDR strains is alarming because antimicrobial treatment against these strains may lead to therapeutic failure. Furthermore, the ERIC- PCR and MLST results suggested that most strains belonged to one main cluster. Thus, a prevalent subtype of Salmonella 1,4, [5],12:i:- strains has probably been circulating among different sources in S & atilde;o Paulo, Brazil, over decades. (AU)

• Processo FAPESP: 19/19338-8 - Genômica, transcriptômica e caracterização fenotipica de linhagens de Campylobacter jejuni isoladas de diversas fontes durante 20 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/07013-0 - Análises do genoma, transcriptoma, construção de mutantes e caracterização fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes durante 25 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular