Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated (1 -> 3)(1 -> 6)-beta-D-Glucan

Autor(es)

Brandi, Jamile ^[1] ; Oliveira, Eder C. ^[1] ; Monteiro, Nilson K. ^[1] ; Vasconcelos, Ana Flora D. ^[1] ; Dekker, Robert F. H. ^[2] ; Barbosa, Aneli M. ^[3] ; Silveira, Joana L. M. ^[4] ; Mourao, Paulo A. S. ^[5] ; Corradi da Silva, Maria de Lourdes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias & Technol, Dept Fis Quim Biol, BR-19060900 Presidente Prudente, SP - Brazil

^[2] Lakehead Univ, Biorefining Res Inst, Thunder Bay, ON P7B 5E1 - Canada

^[3] Univ Estadual Londrina, Dept Bioquim & Biotechnol, BR-86051990 Londrina, Parana - Brazil

^[4] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana - Brazil

^[5] Univ Fed Rio de Janeiro, HU Clementino Fraga Filho, Lab Tecido Conjunt, BR-21941590 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY; v. 21, n. 10, p. 1036-1042, OCT 2011.
• Citações Web of Science: 7

Resumo

The exopolysaccharide botryosphaeran (EPS(GLC); a (1 -> 3)(1 -> 6)-beta-D-glucan from Botryosphaeria rhodina MAMB-05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPS(GLC)-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and (13)C nuclear magnetic resonance ((13)C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant. (AU)

• Processo FAPESP: 05/50936-6 - Sulfatacao do exoplissacarideo produzido pelo botryosphaeria rhodina.
• Beneficiário: Jamile Brandi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/53879-3 - Glucanas fúngicas: caracterização química e determinação de propriedades e parâmetros físico-químicos
• Beneficiário: Maria de Lourdes Corradi Custodio da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular