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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated (1 -> 3)(1 -> 6)-beta-D-Glucan

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Author(s):
Brandi, Jamile [1] ; Oliveira, Eder C. [1] ; Monteiro, Nilson K. [1] ; Vasconcelos, Ana Flora D. [1] ; Dekker, Robert F. H. [2] ; Barbosa, Aneli M. [3] ; Silveira, Joana L. M. [4] ; Mourao, Paulo A. S. [5] ; Corradi da Silva, Maria de Lourdes [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, Fac Ciencias & Technol, Dept Fis Quim Biol, BR-19060900 Presidente Prudente, SP - Brazil
[2] Lakehead Univ, Biorefining Res Inst, Thunder Bay, ON P7B 5E1 - Canada
[3] Univ Estadual Londrina, Dept Bioquim & Biotechnol, BR-86051990 Londrina, Parana - Brazil
[4] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana - Brazil
[5] Univ Fed Rio de Janeiro, HU Clementino Fraga Filho, Lab Tecido Conjunt, BR-21941590 Rio De Janeiro - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY; v. 21, n. 10, p. 1036-1042, OCT 2011.
Web of Science Citations: 7
Abstract

The exopolysaccharide botryosphaeran (EPS(GLC); a (1 -> 3)(1 -> 6)-beta-D-glucan from Botryosphaeria rhodina MAMB-05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPS(GLC)-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and (13)C nuclear magnetic resonance ((13)C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant. (AU)