Autor(es)

Delgado-Garcia, Lina M. ; Ojalvo-Sanz, Ana C. ; Nakamura, Thabatta K. E. ; Martin-Lopez, Eduardo ; Porcionatto, Marimelia ; Lopez-Mascaraque, Laura

• Tipo de documento: Artigo Científico
• Fonte: Biological Research; v. 57, n. 1, p. 16-pg., 2024-08-14.

Resumo

Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: "moderate", "strong," and "very strong". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the "strong" and "very strong" response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair. (AU)

• Processo FAPESP: 19/09183-7 - Caracterização das respostas clonais astrogliais em modelos animais experimentais de lesão cerebral
• Beneficiário: Lina Maria Delgado Garcia
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/19084-8 - Crosstalk entre Wnt, SHH e Notch e o efeito da proteína Gal3 na regulação da proliferação de células-tronco neurais derivadas de astrócitos reativos
• Beneficiário: Lina Maria Delgado Garcia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/12605-8 - Desenvolvimento de microplataformas brain-on-a-chip para modelagem do sistema nervoso central in vitro
• Beneficiário: Marimélia Aparecida Porcionatto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/04258-1 - Adaptações da unidade neurovascular na regulação dos mecanismos de apoptose e resposta glial após lesão cerebral
• Beneficiário: Lina Maria Delgado Garcia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado