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Analytical determination of tryptoquialanines A and B: Ensuring the quality and safety of orange juices

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Autor(es):
Araujo, Eder de Vilhena ; Jesus, Francielly F. Souza ; Valente, Beatriz Lederman ; Cristianini, Marcelo ; Montagner, Cassiana C. ; Fill, Taicia P.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Food Research International; v. 174, p. 8-pg., 2023-10-31.
Resumo

Although orange juice is a popular beverage worldwide, fruit distribution, storage, and processing can facilitate fungal infection by Penicillium digitatum; leading to the production of tremorgenic alkaloids, specifically tryptoquialanines A (TA) and B (TB). An Analytical method was developed and validated based on QuEChERS and LC-MS/MS analysis to determine the levels of TA and TB in fresh, industrial, and homemade orange juices. Excellent linearity was observed in the method over a high range of 1-1000 mu g/kg and low range of 1-75 mu g/kg with R-2 >= 0.998. The LOD and LOQ were 1 and 3 mu g/kg, respectively. Recoveries showed values between 57 and 83 %, with RSD <= 13 %. Our data indicated a higher prevalence of mycotoxin TA in fresh and industrial orange juices. Reduction in TA and TB content after thermal and HPP treatments were <= 32 %. However, thermal treatment was more effective in reducing TA and TB contents. (AU)

Processo FAPESP: 22/02992-0 - Decifrando o papel de metabólitos secundários na interação química entre fitopatógenos e o hospedeiro citros
Beneficiário:Taicia Pacheco Fill
Modalidade de apoio: Auxílio à Pesquisa - Projeto Inicial
Processo FAPESP: 21/00728-0 - Uma abordagem química para entender a doença Huanglongbing (greening)
Beneficiário:Taicia Pacheco Fill
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50951-4 - INCT 2014: Tecnologias Analíticas Avançadas
Beneficiário:Celio Pasquini
Modalidade de apoio: Auxílio à Pesquisa - Temático