Anti-respiratory syncytial virus and anti-herpes simplex virus activity of chemically engineered sulfated fucans from Cystoseira indica

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Jana, Subrata ; Dyna, Andre Luiz ; Pal, Saikat ; Mukherjee, Shuvam ; Bissochi, Isabella Maria Tomaz ; Yamada-Ogatta, Sueli Fumie ; Darido, Maria Laura Goussain ; Oliveira, Danielle Bruna Leal ; Durigon, Edison Luiz ; Ray, Bimalendu ; Faccin-Galhardi, Ligia Carla ; Ray, Sayani

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 337, p. 13-pg., 2024-04-18.

Resumo

Seaweed polysaccharides, particularly sulfated ones, exhibited potent antiviral activity against a wide variety of enveloped viruses, such as herpes simplex virus and respiratory viruses. Different mechanisms of action were suggested, which may range from preventing infection to intracellular antiviral activity, at different stages of the viral cycle. Herein, we generated two chemically engineered sulfated fucans (C303 and C304) from Cystoseira indica by an amalgamated extraction-sulfation procedure using chlorosulfonic acid-pyridine/N,N-dime- N , N-dime- thylformamide and sulfur trioxide-pyridine/N,N-dimethylformamide N , N-dimethylformamide reagents, respectively. These compounds exhibited activity against HSV-1 and RSV with 50 % inhibitory concentration values in the range of 0.75-2.5 mu g/ mL and low cytotoxicity at concentrations up to 500 mu g/mL. The antiviral activities of chemically sulfated fucans (C303 and C304) were higher than the water (C301) and CaCl2 2 extracted (C302) polysaccharides. Compound C303 had a (1,3)-linked fucan backbone and was branched. Sulfates were present at positions C-2, C-4, and C-2,4 of Fucp, p , and C-6 of Galp p residues of this polymer. Compound C304 had a comparable structure but with more sulfates at C-4 of Fucp p residue. Both C303 and C304 were potent antiviral candidates, acting in a dose-dependent manner on the adsorption and other intracellular stages of HSV-1 and RSV replication, in vitro. . (AU)

• Processo FAPESP: 20/06409-1 - Avaliação da resposta imune humoral e da resposta inflamatória em pacientes com diagnóstico confirmado de COVID-19 no Hospital Sírio Libanês e correlação com a severidade da doença
• Beneficiário: Edison Luiz Durigon
• Modalidade de apoio: Auxílio à Pesquisa - Regular